publication . Article . 2014

Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment

Andrew R Pitt;
Open Access English
  • Published: 07 Oct 2014
  • Country: United Kingdom
Abstract
Reversible phosphorylation plays a key role in numerous biological processes. Mass spectrometry-based approaches are commonly used to analyze protein phosphorylation, but such analysis is challenging, largely due to the low phosphorylation stoichiometry. Hence, a number of phosphopeptide enrichment strategies have been developed, including metal oxide affinity chromatography (MOAC). Here, we describe a new material for performing MOAC that employs a magnetite-doped polydimethylsiloxane (PDMS), that is suitable for the creation of microwell array and microfluidic systems to enable low volume, high throughput analysis. Incubation time and sample loading were explo...
Subjects
free text keywords: TA164, Analytical Chemistry, Spectroscopy, Electrochemistry, Biochemistry, Environmental Chemistry
Related Organizations
40 references, page 1 of 3

1 K. A. Resing and N. G. Ahn, FEBS Lett., 2005, 579, 885-889.

2 H. Steen, J. A. Jebanathirajah, J. Rush, N. Morrice and M. W. Kirschner, Mol. Cell. Proteomics, 2006, 5, 172-181.

3 A. Tichy, B. Salovska, P. Rehulka, J. Klimentova, J. Vavrova, J. Stulik and L. J. Hernychova, Proteomics, 2011, 74, 2786- 2797.

4 A. Leitner, TrAC, Trends Anal. Chem., 2010, 29, 177-185.

5 J. D. Dunn, G. E. Reid and M. L. Bruening, Mass Spectrom. Rev., 2010, 29, 29-54.

6 K. Engholm-Keller and M. R. Larsen, J. Proteomics, 2011, 75, 317-328.

7 H. Matsuda, H. Nakamura and T. Nakajima, Anal. Sci., 1990, 6, 911-912.

8 I. Kuroda, Y. Shintani, M. Motokawa, S. Abe and M. Furuno, Anal. Sci., 1990, 20, 1313-1319.

9 M. W. H. Pinkse, P. M. Uitto, M. J. Hilhorst, B. Ooms and A. R. J. Heck, Anal. Chem., 2004, 76, 3935-3943.

10 M. R. Larsen, T. E. Thingholm, O. N. Jensen, P. Roepstorff and T. J. Jorgensen, Mol. Cell. Proteomics, 2005, 5, 873-886.

11 H. C. Hsieh, C. Sheu, F. K. Shi and D. T. Li, J. Chromatogr. A, 2007, 1165, 128-135.

12 S. S. Liang and S. H. Chen, J. Chromatogr. A, 2009, 1216, 2282-2287.

13 M. P. Alcolea and P. R. Cutillas, Methods Mol. Biol., 2010, 658, 111-126.

14 G. Pocsfalvi, M. Cuccurullo, G. Schlosser, S. Scacco, S. Papa and A. Malorni, Mol. Cell. Proteomics, 2007, 6, 231-237.

15 B. Lin, T. Li, Y. Zhao, F. K. Huang, L. Guo and Y. Q. Feng, J. Chromatogr. A, 2008, 1192, 95-102.

40 references, page 1 of 3
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue