publication . Article . 2000

in vitro selected rna cleaving dna enzymes from a combinatorial library are potent inhibitors of hiv 1 gene expression

Open Access
  • Published: 08 Dec 2000 Journal: Biochemical Journal, volume 352, pages 667-673 (issn: 0264-6021, eissn: 1470-8728, Copyright policy)
  • Publisher: Portland Press Ltd.
<jats:p>Selective inactivation of a target gene by antisense mechanisms is an important biological tool to delineate specific functions of the gene product. Approaches mediated by ribozymes and RNA-cleaving DNA enzymes (DNA enzymes) are more attractive because of their ability to catalytically cleave the target RNA. DNA enzymes have recently gained a lot of importance because they are short DNA molecules with simple structures that are expected to be stable to the nucleases present inside a mammalian cell. We have designed a strategy to identify accessible cleavage sites in HIV-1 gag RNA from a pool of random DNA enzymes, and for isolation of DNA enzymes. A pool...
free text keywords: Cell Biology, Biochemistry, Molecular Biology, Deoxyribozyme, In vitro recombination, Biology, DNA/RNA non-specific endonuclease, Base pair, DNA polymerase, biology.protein, DNA, chemistry.chemical_compound, chemistry, Oligonucleotide, Primase
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