publication . Article . 2010

Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil.

Alexandre Jousset; Enrique Lara; Marcell Nikolausz; Hauke Harms; Antonis Chatzinotas;
Open Access
  • Published: 19 Mar 2010
  • Country: Switzerland
Abstract
Ciliates (or Ciliophora) are ubiquitous organisms which can be widely used as bioindicators in ecosystems exposed to anthropogenic and industrial influences. The evaluation of the environmental impact on soil ciliate communities with methods relying on morphology-based identification may be hampered by the large number of samples usually required for a statistically supported, reliable conclusion. Cultivation-independent molecular-biological diagnostic tools are a promising alternative to greatly simplify and accelerate such studies. In this present work a ciliate-specific fingerprint method based on the amplification of a phylogenetic marker gene (i.e. the 18S ...
Subjects
free text keywords: Environmental Engineering, Waste Management and Disposal, Pollution, Environmental Chemistry, Colpodea, biology.organism_classification, biology, Temperature gradient gel electrophoresis, 18S ribosomal RNA, Ribosomal RNA, Ecosystem, Ciliate, Bioindicator, Ecology, Soil test
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publication . Article . 2010

Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil.

Alexandre Jousset; Enrique Lara; Marcell Nikolausz; Hauke Harms; Antonis Chatzinotas;