publication . Article . 2017

методология исследования белково пептидных компонентов экстрактов тканей sus scrofa

Ekaterina R.. Vasilevskaya; Elena A. Kotenkova; Ekaterina A. Lukinova; Evgenia A. Kalinova;
Open Access
  • Published: 01 Jan 2017 Journal: Theory and practice of meat processing, volume 2, pages 79-85 (issn: 2414-438X, eissn: 2414-441X, Copyright policy)
  • Publisher: The Gorbatov's All-Russian Meat Research Institute
Abstract
The article presents a comparative analysis of four methods for quantifying the protein-peptide complexes content in extracts obtained from animal raw materials, as well as the low- and highmolecular weight extract fractions: the direct spectrophotometric determination at wavelengths of 260 and 280 nm with subsequent calculation by the Kalckar formula; the biuret reaction by the Kingsley-Weichselbaum method; the method with Bradford reagent and the standard Lowry method. Experimental data analysis demonstrates that in case of the extract that contains protein-peptidic complexes in different molecular weights range, the Kingsley-Weichselbaum method shows the high...
Subjects
free text keywords: Spectrophotometry, medicine.diagnostic_test, medicine, Peptide bond, Lowry protein assay, Chromatography, Biuret test, Molecular mass, Bradford protein assay, Reagent, Aromatic amino acids, chemistry.chemical_compound, chemistry
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