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Frontiers in Microbiology
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Frontiers in Microbiology
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Endogenous CRISPR-Cas Systems in Group I Clostridium botulinum and Clostridium sporogenes Do Not Directly Target the Botulinum Neurotoxin Gene Cluster

Authors: Travis G. Wentz; Travis G. Wentz; Travis G. Wentz; Benjamin J. M. Tremblay; Marite Bradshaw; Andrew C. Doxey; Shashi K. Sharma; +2 Authors

Endogenous CRISPR-Cas Systems in Group I Clostridium botulinum and Clostridium sporogenes Do Not Directly Target the Botulinum Neurotoxin Gene Cluster

Abstract

Most strains of proteolytic group I Clostridium botulinum (G1 C. botulinum) and some strains of Clostridium sporogenes possess genes encoding botulinum neurotoxin (BoNT), a potent neuroparalytic agent. Within G1 C. botulinum, conserved bont gene clusters of three major toxin serotypes (bont/A/B/F) can be found on conjugative plasmids and/or within chromosomal pathogenicity islands. CRISPR-Cas systems enable site-specific targeting of previously encountered mobile genetic elements (MGE) such as plasmids and bacteriophage through the creation of a spacer library complementary to protospacers within the MGEs. To examine whether endogenous CRISPR-Cas systems restrict the transfer of bont gene clusters across strains we conducted a bioinformatic analysis profiling endogenous CRISPR-Cas systems from 241 G1 C. botulinum and C. sporogenes strains. Approximately 6,200 CRISPR spacers were identified across the strains and Type I-B, III-A/B/D cas genes and CRISPR array features were identified in 83% of the strains. Mapping the predicted spacers against the masked strain and RefSeq plasmid dataset identified 56,000 spacer–protospacer matches. While spacers mapped heavily to targets within bont(+) plasmids, no protospacers were identified within the bont gene clusters. These results indicate the toxin is not a direct target of CRISPR-Cas but the plasmids predominantly responsible for its mobilization are. Finally, while the presence of a CRISPR-Cas system did not reliably indicate the presence or absence of a bont gene cluster, comparative genomics across strains indicates they often occupy the same hypervariable loci common to both species, potentially suggesting similar mechanisms are involved in the acquisition and curation of both genomic features.

Keywords

Microbiology (medical), conjugative plasmids, CRISPR, botulinum, botulinum neurotoxin, horizontal gene transfer, CRISPR-Cas, Microbiology, QR1-502

109 references, page 1 of 11

Alikhan, N.-F., Petty, N. K., Ben Zakour, N. L., and Beatson, S. A. (2011). BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics 12:402. doi: 10.1186/1471-2164-12-402 [OpenAIRE]

Altae-Tran, H., Kannan, S., Demircioglu, F. E., Oshiro, R., Nety, S. P., McKay, L. J., et al. (2021). The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Science 374, 57-65.

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403-410. doi: 10.1016/s0022- 2836(05)80360-2

Arndt, D., Grant, J. R., Marcu, A., Sajed, T., Pon, A., Liang, Y., et al. (2016). PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res. 44, W16-W21. doi: 10.1093/nar/gkw387 [OpenAIRE]

Arnon, S. S., Midura, T. F., Damus, K., Thompson, B., Wood, R. M., and Chin, J. (1979). Honey and other environmental risk factors for infant botulism. J. Pediatr. 94, 331-336. doi: 10.1016/s0022-3476(79)80863-x

Bhaya, D., Davison, M., and Barrangou, R. (2011). CRISPR-Cas Systems in Bacteria and Archaea: versatile Small RNAs for Adaptive Defense and Regulation. Annu. Rev. Genet. 45, 273-297. doi: 10.1146/annurev-genet-110410-132430 [OpenAIRE]

Biswas, A., Gagnon, J. N., Brouns, S. J. J., Fineran, P. C., and Brown, C. M. (2013). CRISPRTarget. RNA Biol. 10, 817-827. doi: 10.4161/rna.24046

Brooks, L., Kaze, M., and Sistrom, M. (2019). A Curated, Comprehensive Database of Plasmid Sequences. Microbiol. Resour. Announc. 8, e01325-18. doi: 10.1128/ mra.01325-18

Brunt, J., Carter, A. T., Stringer, S. C., and Peck, M. W. (2018). Identification of a novel botulinum neurotoxin gene cluster in Enterococcus. FEBS Lett. 592, 310-317. doi: 10.1002/1873-3468.12969

Brunt, J., Van Vliet, A. H. M., Stringer, S. C., Carter, A. T., Lindström, M., and Peck, M. W. (2020b). Pan-Genomic Analysis of Clostridium botulinum Group II (Non-Proteolytic C. botulinum) Associated with Foodborne Botulism and Isolated from the Environment. Toxins 12:306. doi: 10.3390/toxins12050306

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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    5
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Top 10%
Average
Average
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NIH| Characteristics of Botulinum Neurotoxins that determine potency
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R01AI139306-01A1
  • Funding stream: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
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