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Publication . Article . Other literature type . 2011

Microbial based assay for specific detection of β-lactam group of antibiotics in milk

Sougata Das; Naresh Kumar; Raghu Hirikyathanahalli Vishweswaraiah; Lopamudra Haldar; Manju Gaare; V. K. Singh; Anil Kumar Puniya;
Open Access
Published: 24 Dec 2011
Publisher: Springer India
The spore forming Bacillus cereus (66) was screened for the induction of β-lactamase in presence of an inducer using iodometric assay. A significant induction in marker enzyme was observed in B. cereus 66 at maximum residual limit (MRL) of penicillin, ampicillin, cloxacillin, amoxicillin, cefalexin, and cephazolin belonging to β-lactam group of antibiotics. A microbial based assay, where enzyme induction was optimized at pH 7.0, temperature 30°C, and whey powder (0.25%) after 4 h of incubation. The spore based assay was tested with milk samples spiked with 6 different β-lactam antibiotics. The results were 100 and 83.33% in correlation with microbial receptor and inhibition based assay, respectively. Overall, spore based assay can be a useful and cost effective tool for the specific detection of β-lactam group of antibiotics in milk.
Subjects by Vocabulary

Medical Subject Headings: fungi polycyclic compounds

Microsoft Academic Graph classification: Microbiology Chromatography Biology Enzyme inducer biology.protein Cloxacillin medicine.drug medicine Antibiotics medicine.drug_class Cereus biology.organism_classification Bacillus cereus Ampicillin Cefalexin Penicillin


Original Article, Food Science