Bicycle mobility has become increasingly popular as a sustainable and healthy means of transportation. Bicycles are not only a cost-effective transportation mode but also help reduce traffic congestion and air pollution. However, the efficiency and safety of bicycling largely depend on the optimization of bicycle components, such as the tires. The importance of bike tire optimization cannot be underestimated as it can affect both bicycle dynamics and bicycle performance. Due to the lack of multi-physical mathematical models able to analyze and reproduce complex tire/road contact phenomena, useful to predict the wide range of working conditions, this research aims to the development of a bicycle tire thermal model. The main outcome is to provide the full temperature local distribution inside the tire’s inner rubber layers and the inflation chamber. Such kind of information plays a fundamental role in the definition of the optimal adherence conditions, for both safety and performance maximization, and as an indicator of the proper tire design for various applications, each requiring specific heat generation and management. The experimental validation has been carried out thanks to an innovative test-rig developed at Politecnico di Milano. It is known as VetyT (acronym of Velo Tyre Testing), and it complies with the standard ISO 9001-2015. It has been specifically instrumented for the activity, acquiring the external tire temperatures to be compared with the respective simulated ones, under various workingconditions.