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Virology
Article . 2014
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Defining the roles of the baculovirus regulatory proteins IE0 and IE1 in genome replication and early gene transactivation

Authors: Sokal, Nadia; Nie, Yingchao; Willis, Leslie G.; Yamagishi, Junya; Blissard, Gary W.; Rheault, Mark R.; Theilmann, David A.;

Defining the roles of the baculovirus regulatory proteins IE0 and IE1 in genome replication and early gene transactivation

Abstract

AbstractIE0 and IE1 of the baculovirus Autographa californica multiple nucleopolyhedrovirus are essential transregulatory proteins required for both viral DNA replication and transcriptional transactivation. IE0 is identical to IE1 except for 54 amino acids at the N-terminus but the functional differences between these two proteins remain unclear. The purpose of this study was to determine the separate roles of these critical proteins in the virus life cycle. Unlike prior studies, IE0 and IE1 were analyzed using viruses that expressed ie0 and ie1 from an identical promoter so that the timing and levels of expression were comparable. IE0 and IE1 were found to equally support viral DNA replication and budded virus (BV) production. However, specific viral promoters were selectively transactivated by IE0 relative to IE1 but only when expressed at low levels. These results indicate that IE0 preferentially transactivates specific viral genes at very early times post-infection enabling accelerated replication and BV production.

Subjects by Vocabulary

Microsoft Academic Graph classification: Biology Transactivation Replication factor C Viral life cycle Control of chromosome duplication Gene Genetics DNA replication Promoter Origin recognition complex

Keywords

Gene Expression Regulation, Viral, Transcriptional Activation, Transcription, Genetic, IE1, DNA replication, Moths, Virus Replication, IE0, Cell Line, Immediate-Early Proteins, Virology, Animals, Baculovirus, Transactivation, AcMNPV, Trans-Activators, Gene expression, Transcription factor, Baculoviridae, Plasmids

68 references, page 1 of 7

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  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    8
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
8
Average
Average
Average
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  • Funder: Natural Sciences and Engineering Research Council of Canada (NSERC)
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