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Lipid transfer between human plasma low-density lipoprotein and a triolein/phospholipid microemulsion catalyzed by insect hemolymph lipid transfer particle
Copyright policy )Lipid transfer between human plasma low-density lipoprotein and a triolein/phospholipid microemulsion catalyzed by insect hemolymph lipid transfer particle
Abstract Lipid transfer between human plasma low-density lipoprotein (LDL) and an LDL-size microemulsion of triolein and phosphatidylcholine stabilized with human apolipoprotein A-I was catalyzed by the lipid transfer particle from hemolymph of the tobacco hornworm (Manduca sexta). Net transfer of phospholipid and triacylglycerol from the emulsion to LDL was observed and the apparent initial rates of transfer were dependent on the amount of catalyst. Net transfer of phospholipid mass was twice as much as that of triacylglycerol with respect to both the initial rate and the final equilibrium state. The final amount of net transfer of both lipids was dependent upon the initial ratio of LDL : microemulsion present in the incubation mixture up to 1:1 on the basis of phospholipid. The microemulsion lipid composition was maximally altered from an initial weight ratio of 1.09 ± 0.08 (phospholipid/triolein) to 0.90 ± 0.03 by this reaction. Further increase of LDL in the incubation caused neither further net transfer nor further change in the lipid composition of the microemulsion. The catalyst neither affected spontaneous transfer of free cholesterol between the emulsion and LDL nor enhanced cholesteryl ester transfer in this reaction system. As a result of the facilitated reaction, LDL gained a significant amount of phospholipid and triacylglycerol causing up to an 8% increase in core lipids and 14% in phospholipid. Some free cholesterol is recovered in the emulsions via spontaneous exchange. Transfer or exchange of apolipoproteins during the course of facilitated lipid transfer did not occur.
- University of Alberta Canada
Microsoft Academic Graph classification: Phospholipid Biology chemistry.chemical_compound Phosphatidylcholine Triolein Chromatography Triglyceride Cholesterol chemistry Low-density lipoprotein Cholesteryl ester Lipoprotein
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
Biophysics, In Vitro Techniques, Moths, Biochemistry, Endocrinology, Hemolymph, Animals, Humans, Apolipoproteins A, Phospholipids, Apolipoprotein A-I, Lipoproteins, LDL, Cholesterol, Emulsions, Cholesterol Esters, Carrier Proteins, Triolein
Biophysics, In Vitro Techniques, Moths, Biochemistry, Endocrinology, Hemolymph, Animals, Humans, Apolipoproteins A, Phospholipids, Apolipoprotein A-I, Lipoproteins, LDL, Cholesterol, Emulsions, Cholesterol Esters, Carrier Proteins, Triolein
Microsoft Academic Graph classification: Phospholipid Biology chemistry.chemical_compound Phosphatidylcholine Triolein Chromatography Triglyceride Cholesterol chemistry Low-density lipoprotein Cholesteryl ester Lipoprotein
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
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Citations provided by BIP!Popularity provided by BIP!- University of Alberta Canada
Abstract Lipid transfer between human plasma low-density lipoprotein (LDL) and an LDL-size microemulsion of triolein and phosphatidylcholine stabilized with human apolipoprotein A-I was catalyzed by the lipid transfer particle from hemolymph of the tobacco hornworm (Manduca sexta). Net transfer of phospholipid and triacylglycerol from the emulsion to LDL was observed and the apparent initial rates of transfer were dependent on the amount of catalyst. Net transfer of phospholipid mass was twice as much as that of triacylglycerol with respect to both the initial rate and the final equilibrium state. The final amount of net transfer of both lipids was dependent upon the initial ratio of LDL : microemulsion present in the incubation mixture up to 1:1 on the basis of phospholipid. The microemulsion lipid composition was maximally altered from an initial weight ratio of 1.09 ± 0.08 (phospholipid/triolein) to 0.90 ± 0.03 by this reaction. Further increase of LDL in the incubation caused neither further net transfer nor further change in the lipid composition of the microemulsion. The catalyst neither affected spontaneous transfer of free cholesterol between the emulsion and LDL nor enhanced cholesteryl ester transfer in this reaction system. As a result of the facilitated reaction, LDL gained a significant amount of phospholipid and triacylglycerol causing up to an 8% increase in core lipids and 14% in phospholipid. Some free cholesterol is recovered in the emulsions via spontaneous exchange. Transfer or exchange of apolipoproteins during the course of facilitated lipid transfer did not occur.