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FISH Variants

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Other literature type 13 Feb 2021Springer US

Nuno M. Guimarães; Nuno F. Azevedo; Carina Almeida;

doi: 10.1007/978-1-0716-1115-9_2

handle: 1822/74508

FISH Variants

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Abstract

FISH has gained an irreplaceable place in microbiology because of its ability to detect and locate a microorganism, or a group of organisms, within complex samples. However, FISH role has evolved drastically in the last few decades and its value has been boosted by several advances in signal intensity, imaging acquisitions, automation, method robustness, and, thus, versatility. This has resulted in a range of FISH variants that gave researchers the ability to access a variety of other valuable information such as complex population composition, metabolic activity, gene detection/quantification, or subcellular location of genetic elements. In this chapter, we will review the more relevant FISH variants, their intended use, and how they address particular challenges of classical FISH.

Financially supported by (a) Base Funding—UIDB/ 00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology, and Energy—LEPABE—funded by national funds through the FCT/MCTES (PIDDAC); (b) Projects POCI01-0145-FEDER-016678 (Coded-FISH), POCI-01-0145- FEDER-029841 (POLY-PREVENTT), and POCI-01-0145- FEDER-029961 (ColorISH), funded by FEDER funds through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT/MCTES; (c) BioTecNorte operation (NORTE-01- 0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte

info:eu-repo/semantics/publishedVersion

Country

Portugal

Related Organizations

University of Minho
Portugal

Keywords

FISH, CARD-FISH, Gene-FISH, Phage-FISH, MAR-FISH, FISH-NanoSIMS, CLASI-FISH, DOPE-FISH, Leaf-FISH, Flow-FISH, Microfluidic-FISH, NAM-FISH QD-FISH, NAM-FISH, QD-FISH, Ciências Agrárias::Biotecnologia Agrária e Alimentar

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	2
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average

Usage byUsageCounts

visibility	views	9
download	downloads	1

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	2
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average