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Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
Copyright policy )Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.
- University of Bonn Germany
- Robert Koch Institute Germany
- University of Duisburg-Essen Germany
- Erasmus University Rotterdam Netherlands
Microsoft Academic Graph classification: Sequence analysis Immunofluorescence Microscopy Biology chemistry.chemical_compound Polymorphism (computer science) Gene Human coronavirus Molecular biology Virology Highly sensitive Antibody response chemistry DNA
Epidemiology, Medizin, Fluorescent Antibody Technique, Sensitivity and Specificity, Germany, Virology, Humans, Reverse Transcriptase Polymerase Chain Reaction, Public Health, Environmental and Occupational Health, Sequence Analysis, DNA, Coronavirus, RNA, Viral, Coronavirus Infections, Laboratories, Polymorphism, Restriction Fragment Length
Epidemiology, Medizin, Fluorescent Antibody Technique, Sensitivity and Specificity, Germany, Virology, Humans, Reverse Transcriptase Polymerase Chain Reaction, Public Health, Environmental and Occupational Health, Sequence Analysis, DNA, Coronavirus, RNA, Viral, Coronavirus Infections, Laboratories, Polymorphism, Restriction Fragment Length
Microsoft Academic Graph classification: Sequence analysis Immunofluorescence Microscopy Biology chemistry.chemical_compound Polymorphism (computer science) Gene Human coronavirus Molecular biology Virology Highly sensitive Antibody response chemistry DNA
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).317 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1% citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).317 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!
- University of Bonn Germany
- Robert Koch Institute Germany
- University of Duisburg-Essen Germany
- Erasmus University Rotterdam Netherlands
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.