<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Claim

Promising Prebiotic Candidate Established by Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2018 Netherlands, Canada American Society for MicrobiologyApplied and Environmental Microbiology, volume 84 (issn: 0099-2240, eissn: 1098-5336,

Authors: Stephanie L. Collins; Amy McMillan; Shannon Seney; Charlotte van der Veer; Remco Kort; Mark W. Sumarah; Gregor Reid;

doi: 10.1128/aem.02200-17

pmid: 29269494

pmc: PMC5812932

handle: 1871.1/21929fc7-58a8-4fc6-8283-bb41ac83dc9c

Promising Prebiotic Candidate Established by Evaluation of Lactitol, Lactulose, Raffinose, and Oligofructose for Maintenance of a Lactobacillus-Dominated Vaginal Microbiota

- Summary
- Subjects
- References
  (59)
- Related research
  (10)
- Metrics

Abstract

ABSTRACT Perturbations to the vaginal microbiota can lead to dysbiosis, including bacterial vaginosis (BV), which affects a large portion of the female population. In a healthy state, the vaginal microbiota is characterized by low diversity and colonization by Lactobacillus spp., whereas in BV, these species are displaced by a highly diverse population of bacteria associated with adverse vaginal health outcomes. Since prebiotic ingestion has been a highly effective approach to invigorate lactobacilli for improved intestinal health, we hypothesized that these compounds could stimulate lactobacilli at the expense of BV organisms to maintain vaginal health. Monocultures of commensal Lactobacillus crispatus , Lactobacillus vaginalis , Lactobacillus gasseri , Lactobacillus johnsonii , Lactobacillus jensenii , and Lactobacillus iners , in addition to BV-associated organisms and Candida albicans , were tested for their ability to utilize a representative group of prebiotics consisting of lactitol, lactulose, raffinose, and oligofructose. The disaccharide lactulose was found to most broadly and specifically stimulate vaginal lactobacilli, including the strongly health-associated species L. crispatus , and importantly, not to stimulate BV organisms or C. albicans . Using freshly collected vaginal samples, we showed that exposure to lactulose promoted commensal Lactobacillus growth and dominance and resulted in healthy acidity partially through lactic acid production. This provides support for further testing of lactulose to prevent dysbiosis and potentially to reduce the need for antimicrobial agents in managing vaginal health. IMPORTANCE Bacterial vaginosis (BV) and other dysbioses of the vaginal microbiota significantly affect the quality of life of millions of women. Antimicrobial therapy is often poorly effective, causes side effects, and does not prevent recurrences. We report one of very few studies that have evaluated how prebiotics—compounds that are selectively fermented by beneficial bacteria such as Lactobacillus spp.—can modulate the vaginal microbiota. We also report use of a novel in vitro polymicrobial model to study the impact of prebiotics on the vaginal microbiota. The identification of prebiotic lactulose as enhancing Lactobacillus growth but not that of BV organisms or Candida albicans has direct application for retention of homeostasis and prevention of vaginal dysbiosis and infection.

Countries

Netherlands, Canada

Related Organizations

Universidad Univer
Mexico
Bellevue College
United States
Vrije Universiteit Amsterdam
Netherlands
Western University
Canada
TNO
Netherlands

View all View all

Subjects by Vocabulary

Microsoft Academic Graph classification: Lactobacillus Lactobacillus crispatus biology Lactobacillus vaginalis Lactobacillus gasseri Microbiology medicine Lactobacillus iners Lactobacillus johnsonii Lactobacillus jensenii biology.organism_classification medicine.disease Dysbiosis

Medical Subject Headings: food and beverages

Keywords

oligofructose, Biomedical Innovation, Oligosaccharides, Applied Microbiology and Biotechnology, Mass Spectrometry, Sugar Alcohols, Lactobacillus gasseri, Life, RNA, Ribosomal, 16S, Candida albicans, Lactobacillus crispatus, Lactitol, Bacteria (microorganisms), Candida, Ecology, Microbiota, Lactobacillus vaginalis, Lactic acid, Bacterial vaginosis, Lactulose, Oligo-fructose, RNA, Bacterial, Health, Vagina, Female, Vaginal microbiota, Lactobacillus jensenii, Healthy Living, bacterial vaginosis, Microbiotas, Biotechnology, lactobacilli, raffinose, Oligofructose, lactitol, Bacilli, Microbial Ecology, Raffinose, Humans, Metabolomics, Biology, Lactobacillus johnsonii, vaginal microbiota, Bacteria, Sequence Analysis, RNA, Antimicrobial agents, Lactobacillus, MSB - Microbiology and Systems Biology, Prebiotics, Lactobacilli, Dysbiosis, lactulose, ELSS - Earth, Life and Social Sciences, Lactobacillus iners, Food Science

59 references, page 1 of 6

1.Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SSK, McCulle SL, Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ 2011 Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci U S A 108:4680–4687. doi:10.1073/pnas.1002611107.20534435 [OpenAIRE] [PubMed] [DOI]

2.Reid G, Younes JA, Van der Mei HC, Gloor GB, Knight R, Busscher HJ 2011 Microbiota restoration: natural and supplemented recovery of human microbial communities. Nat Rev Microbiol 9:27–38. doi:10.1038/nrmicro2473.21113182 [OpenAIRE] [PubMed] [DOI]

3.Reid G, Brigidi P, Burton JP, Contractor N, Duncan S, Fargier E, Hill C, Lebeer S, Martín R, McBain AJ, Mor G, O'Neill C, Rodríguez JM, Swann J, van Hemert S, Ansell J 2015 Microbes central to human reproduction. Am J Reprod Immunol 73:1–11. doi:10.1111/aji.12319. [OpenAIRE] [DOI]

4.Hummelen R, Fernandes AD, Macklaim JM, Dickson RJ, Changalucha J, Gloor GB, Reid G 2010 Deep sequencing of the vaginal microbiota of women with HIV. PLoS One 5:e12078. doi:10.1371/journal.pone.0012078.20711427 [OpenAIRE] [PubMed] [DOI]

5.Dols JAM, Smit PW, Kort R, Reid G, Schuren FHJ, Tempelman H, Bontekoe TR, Korporaal H, Boon ME 2011 Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis. Am J Obstet Gynecol 204:305.e1–305.e7. doi:10.1016/j.ajog.2010.11.012.21272848 [OpenAIRE] [PubMed] [DOI]

6.McMillan A, Rulisa S, Sumarah M, Macklaim JM, Renaud J, Bisanz JE, Gloor GB, Reid G 2015 A multi-platform metabolomics approach identifies highly specific biomarkers of bacterial diversity in the vagina of pregnant and non-pregnant women. Sci Rep 5:14174. doi:10.1038/srep14174.26387596 [OpenAIRE] [PubMed] [DOI]

7.Ness RB, Kip KE, Hillier SL, Soper DE, Stamm CA, Sweet RL, Rice P, Richter HE 2005 A cluster analysis of bacterial vaginosis-associated microflora and pelvic inflammatory disease. Am J Epidemiol 162:585–590. doi:10.1093/aje/kwi243.16093289 [OpenAIRE] [PubMed] [DOI]

8.Martin HL, Richardson BA, Nyange PM, Lavreys L, Hillier SL, Chohan B, Mandaliya K, Ndinya-Achola JO, Bwayo J, Kreiss J 1999 Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J Infect Dis 180:1863–1868. doi:10.1086/315127.10558942 [OpenAIRE] [PubMed] [DOI]

9.Wiesenfeld HC, Hillier SL, Krohn MA, Landers DV, Sweet RL 2003 Bacterial vaginosis is a strong predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection. Clin Infect Dis 36:663–668. doi:10.1086/367658.12594649 [OpenAIRE] [PubMed] [DOI]

10.Redondo-Lopez V, Meriwether C, Schmitt C, Opitz M, Cook R, Sobel JD 1990 Vulvovaginal candidiasis complicating recurrent bacterial vaginosis. Sex Transm Dis 17:51–53. doi:10.1097/00007435-199001000-00011.2305336 [OpenAIRE] [PubMed] [DOI]

chevron_left
1
2
3
4
5
chevron_right

10 Research products, page 1 of 1

A Genomic View of Lactobacilli and Pediococci Demonstrates that Phylogeny Matches Ecology and Physiology
2015IsAmongTopNSimilarDocuments
Phylotype-Level Profiling of Lactobacilli in Highly Complex Environments by Means of an Internal Transcribed Spacer-Based Metagenomic Approach
2018IsAmongTopNSimilarDocuments
Molecular Analysis and Clinical Significance of Lactobacillus spp. Recovered from Clinical Specimens Presumptively Associated with Disease
2014IsAmongTopNSimilarDocuments
Prokaryotic communities in pit mud from different-aged cellars used for the production of Chinese strong-flavored liquor.
2014IsAmongTopNSimilarDocuments
Lactobacillus reuteri 100-23 modulates urea hydrolysis in the murine stomach
2014IsAmongTopNSimilarDocuments
Association between Obesity and Cervical Microflora Dominated by Lactobacillus iners in Korean Women
2015IsAmongTopNSimilarDocuments
Priming of the Respiratory Tract with Immunobiotic Lactobacillus plantarum Limits Infection of Alveolar Macrophages with Recombinant Pneumonia Virus of Mice (rK2-PVM)
2016IsAmongTopNSimilarDocuments
A Lactobacillus-Deficient Vaginal Microbiota Dominates Postpartum Women in Rural Malawi
2018IsAmongTopNSimilarDocuments
Changes in the Rumen Epimural Bacterial Diversity of Beef Cattle as Affected by Diet and Induced Ruminal Acidosis
2013IsAmongTopNSimilarDocuments
Inhibition of Staphylococcus aureus by Lysostaphin-Expressing Lactobacillus plantarum WCFS1 in a Modified Genital Tract Secretion Medium
2011IsAmongTopNSimilarDocuments

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	54
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 1%

Usage byUsageCounts

visibility

views

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	54
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 1%