One of the main factors in the regulation of the menstrual cycle is prolactin, the level of which begins to change actively with the onset of puberty. Anxiety is one of the main characteristics of a person’s psychological profile. Therefore, by acting on the level of anxiety, we can neutralize the effect of chronic and/or acute stress on the hypothalamic-pituitary system, including the level of prolactin.The objective: was to determine the correlations between different levels of anxiety and the presence of hyperprolactinemia, to study the possibilities of correcting the level of prolactin by means of complex anti-stress therapy.Materials and methods. To achieve this goal, we conducted a survey of 94 adolescent girls during the period of the formation of menstrual function. To study the psychoemotional state of the patients, the Spielberger-Hanin anxiety scale was used.Results. Among girls 14.0±1.5 years old with hyperprolactinemia, the presence of acute and chronic stress was revealed in 92.2% of cases. Before treatment, the proportion of high personal anxiety in girls with hyperprolactinemia was 4 times higher, and low personal anxiety was 6 times lower than in healthy women. The proportion of high situational anxiety significantly exceeded this indicator in healthy girls, moderate situational anxiety in girls with hyperprolactinemia was 3 times lower than in healthy women. And the proportion of low situational anxiety in girls with hyperprolactinemia was almost 5 times lower than in healthy women. Three months after the start of treatment, the proportion of high and moderate personal anxiety in girls with hyperprolactinemia decreased slightly, and the proportion of low personal anxiety increased significantly after the psychological correction. In contrast to personal anxiety, situational anxiety depends on living conditions, therefore, its changes were significant after anti-stress treatment. But even without psychological correction, positive changes were noted in girls with hyperprolactinemia. After treatment, the level of prolactin on average in girls with hyperprolactinemia decreased by 49.4% and reached normal values, while in girls without treatment, the level of prolactin decreased by only 20.5% and its level remained slightly above the upper limit of the norm.Conclusion. The fact that 92.2% of girls with hyperprolactinemia have acute or chronic stress and high situational anxiety (70.3%) and high personal anxiety (39.1%) indicates a direct correlation between high levels of anxiety and the presence of hyperprolactinemia, which confirms the need for conducting complex anti-stress therapy in girls with hyperprolactinemia.