This paper presents the results of the analysis of acetone, chloroform and diethyl ether extracts of three types of wormwood: Artemisia absinthium L., Artemisia armeniaca Lam. and Artemisia latifolia Ledeb., performed by gas chromatography–mass spectroscopy. β-myrcene was found in chloroform extracts of A. absinthium and A. latifolia from biologically active substances; in extracts of A. armeniaca and A. latifolia, pinene; 3,6-difluoro-4-[1-hydroxy-2-(methiamino)ethyl]benzene-1,2-diol, ethyl palmitate, β-phellandrene are specific for A. absinthium; for A. armeniaca, herniarin and d-limonene; for A. latifolia, γ-sitosterol and farnesol. In acetone extracts of all three types, such biologically active substances as γ-sitosterol, phytol were found; extracts of A. absinthium and A. armeniaca revealed α-cubeben; in extracts of A. armeniaca and A. latifolia – vitamin E; in extracts of A. absinthium and A. latifolia – azafrin. Specific for these extracts of A. absinthium were β-phellandrene, estafiantin, β-elemen, yangambin, β-santalol, caryophyllene, lycopene; for A. armeniaca, α-phellandrene and γ-element; for A. latifolia – hematoporphyrin and laminitol. In the diethyl ether extract of A. absinthium, such biologically active substances as arborescin and yangambin were found; in the extract of A. armeniaca – D-limonene; A. latifolia extract contains hematoporphyrin. Based on the data obtained, it should be assumed that preparations of these plant species may have antibacterial, antioxidant, and anti-inflammatory activity.