The application of the Universal Design for Learning framework favors the creation of virtual educational environments for all. It requires the development of accessible content, a usable platform and the use of flexible didactics and evaluations that promote the constant motivation of students. The present study aims to design a methodology to evaluate the usability of the Moodle platform based on the principles of Universal Design for Learning. It recognizes the importance of accessibility, usability and the availability of Assistive Technologies in an inclusive virtual education that meets the needs of students. We developed and applied a methodology to assess the level of usability of Moodle, taking into account that they integrate Assistive Technologies or are used for MOOC contexts. We employ the mixed design research framework to evaluate a MOOC-type educational program. We conclude that Universal Design for Learning principles and guidelines are positively valued by students and have a positive impact on certification rates.