Clinical evaluation of a multiplex PCR-based test for joint infection: a prospective diagnostic accuracy study of forty-nine patients
Copyright policy )Clinical evaluation of a multiplex PCR-based test for joint infection: a prospective diagnostic accuracy study of forty-nine patients
Abstract Purpose The purpose of this study was to evaluate the diagnostic accuracy (sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV)) of the PCR-based BioFire® Joint Infection Panel (BJI Panel) against microbiological culture growth for patients suspected of having a native or prosthetic joint infection. Methods Synovial fluid and tissue biopsies were prospectively collected from patients from June 2022 to June 2023. The results of the BJI Panel were compared with those of culture growth. Results 51 samples were included. Including all pathogens, the sensitivity was 69%, the specificity 89%, the PPV 73% and the NPV 86%. Including only pathogens in the BJI Panel, the sensitivity was 100%, the specificity 90%, the PPV 73% and the NPV 100%. Conclusion The BJI Panel has a high accuracy for detecting the pathogens in its panel, but the absence of important common pathogens from the panel reduces its sensitivity and NPV. With a short turnaround time and precise pathogen detection, the BJI Panel has the potential to add value as a complementary diagnostic method.
- University of Copenhagen Denmark
- University of copenhaguen Denmark
- University of Copenhagen Denmark
- University of Copenhagen, Faculty of Health and Medical Sciences Denmark
- Rigshospitalet Denmark
Male, Adult, Prosthesis-Related Infections, Multiplex Polymerase Chain Reaction/methods, Biopsy, Pathogen detection, Diagnostic accuracy, Sensitivity and Specificity, Joint infection, Prosthesis-Related Infections/diagnosis, Predictive Value of Tests, Synovial Fluid, 80 and over, Humans, Culture growth, Prospective Studies, Infectious/diagnosis, Aged, Aged, 80 and over, Arthritis, Infectious, Arthritis, Middle Aged, PCR, Synovial Fluid/microbiology, Original Article, Female, Multiplex Polymerase Chain Reaction
Male, Adult, Prosthesis-Related Infections, Multiplex Polymerase Chain Reaction/methods, Biopsy, Pathogen detection, Diagnostic accuracy, Sensitivity and Specificity, Joint infection, Prosthesis-Related Infections/diagnosis, Predictive Value of Tests, Synovial Fluid, 80 and over, Humans, Culture growth, Prospective Studies, Infectious/diagnosis, Aged, Aged, 80 and over, Arthritis, Infectious, Arthritis, Middle Aged, PCR, Synovial Fluid/microbiology, Original Article, Female, Multiplex Polymerase Chain Reaction
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