Some of one of the urgent tasks of modern medicine and pharmaceutics, as well as the chemical industry, is the search for and research of natural sources of substances possessing pharmacological activity. Within the framework of these tasks, there is increased interest in medicinal plant raw materials, which is associated with the content of a rich complex of biologically active substances, many of which exhibit specific pharmacological activity. This article is devoted to a comprehensive study of the properties of plant raw materials. As objects of research we used dry capsulated food extract and supercritical fluid CO2-extract of Rhodiola rosea (Rhododiola rosea L.). The following studies were carried out studies of antiradical activity by means of free radical DPPH (2,2-diphenyl-1-propylpropylhydrazyl), antibacterial activity by the hole-diffusion method in the author's modification, analysis of the total content of polyphenolic compounds and flavonoids by spectrophotometry, assessment of mononuclear cell viability using the resazurin test, qualitative analysis of samples by HPLC-MS and UV spectroscopy. It was experimentally revealed that extracts of Rhodiola rosea contain in their composition compounds of the class of polyphenols, flavonoids, glycosides, phytosteroids and terpenoids. CO2-extract has the highest content of polyphenolic compounds, flavonoids - dry extract. Both extracts show antiradical activity, but the dry extract has it to a greater extent. The supercritical fluid extract does not adversely affect the the viability of mononuclear cells. The antibacterial activity of the extracts has not been established. The value of the work is to enrich the knowledge base on the properties of Rhodiola rosea extracts, obtaining new data on its supercritical fluid CO2-extracts, not presented to date among scientific papers. Based on experimental data, it is possible to note the prospective application of Rhodiola extracts in therapeutic and prophylactic treatment of a wide range of action.