Purpose of review Sexually transmitted infections (STIs) are a significant global health concern, with many cases going undiagnosed due to asymptomatic infections. Traditional diagnostic methods, such as culture and serology, have limitations in sensitivity, specificity, and turnaround time. Molecular diagnostics, particularly PCR-based approaches, offer significant advantages, including improved detection rates and the potential for syndromic testing. This review examines the role of syndromic PCR diagnostics in improving STI detection and management. Recent findings Recent studies highlight the utility in detecting common STIs, such as Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis, as well as emerging pathogens. PCR-based syndromic panels allow for the simultaneous detection of multiple pathogens from a single sample, improving diagnostic accuracy and efficiency. Syndromic PCR approaches streamline diagnosis, aid in early detection, and support efficient treatment, addressing both common and emerging infections. Summary Syndromic PCR diagnostics streamline STI detection, addressing the limitations of conventional methods. They enable faster, more accurate, and comprehensive diagnosis, leading to targeted treatment and improved patient outcomes. Expanding syndromic panels to include emerging pathogens and ensuring cost-effective implementation remain key areas for future research.