AbstractScience education at different levels can be supported by various mobile applications that can be downloaded for free onto mobile phones, tablets, and other devices. Such applications can also be used in laboratory work, but it must be said that their use in science laboratories is a relatively new approach. This study is aimed at determining the impact of mobile phone and mobile application-supported laboratory work on the digital literacy of preservice science teachers and on their disposition toward using technology in class. The study was carried out with 17 participants at the second-year level of a Science Teacher Education Program in Turkey. It was a single-group pretest-posttest study, representing a type of weak experimental research design. Data for the study were collected with the Digital Literacy Scale developed by Hamutoğlu et al. (Ege Journal of Education 18(1):408–429, 2017) and the Disposition toward Using Technology in the Classroom Scale developed by Gunuc and Kuzu (Journal of Theory and Practice in Education 10(4):863–884, 2014). The data collection tools were implemented prior to and following a 6-week teaching period, and the quantitative data compiled from the participants were analyzed with the SPSS 21.0 program. The parametric t-test of related samples was used in the pretest/posttest comparison of the data sets. The results of the study demonstrated that science laboratory activities supported by mobile phones and mobile applications provided preservice teachers with increased digital literacy and enhanced their disposition toward using technology in the classroom. It is recommended that preservice teachers make extensive use of experiments supported by the mobile applications in this study.