An integrative genomics approach for deciphering the complex interactions between ascorbate metabolism and fruit growth and composition in tomato
Copyright policy )An integrative genomics approach for deciphering the complex interactions between ascorbate metabolism and fruit growth and composition in tomato
Very few reports have studied the interactions between ascorbate and fruit metabolism. In order to get insights into the complex relationships between ascorbate biosynthesis/recycling and other metabolic pathways in the fruit, we undertook a fruit systems biology approach. To this end, we have produced tomato transgenic lines altered in ascorbate content and redox ratio by RNAi-targeting several key enzymes involved in ascorbate biosynthesis (2 enzymes) and recycling (2 enzymes). In the VTC (ViTamin C) Fruit project, we then generated phenotypic and genomic (transcriptome, proteome, metabolome) data from wild type and mutant tomato fruit at two stages of fruit development, and developed or implemented statistical and bioinformatic tools as a web application (named VTC Tool box) necessary to store, analyse and integrate experimental data in tomato. By using Kohonen's self-organizing maps (SOMs) to cluster the biological data, pair-wise Pearson correlation analyses and simultaneous visualization of transcript/protein and metabolites (MapMan), this approach allowed us to uncover major relationships between ascorbate and other metabolic pathways.
Complex systems, VTC: Vitamin C, Proteome, [SDV]Life Sciences [q-bio], MESH: Ascorbate Oxidase, MESH: Plants, Ascorbic Acid, AsA: Ascorbic acid, NADPH Oxidoreductases, 630, Biologie intégrative, MDHAR, Solanum lycopersicum, MESH: Lycopersicon esculentum, BIOLOGIE CELLULAIRE, MESH: Ascorbic Acid, AO: Ascorbate oxidase, NADH, NADPH Oxidoreductases, CELL WALL, ASCORBIQUE ACID, Plant Proteins, MESH: Plant Proteins, Monodehydroascorbate reductase Ascorbic acid, MESH: Genomics, Cell wall, Genomics, MESH: Carbohydrate Epimerases, Plants, Genetically Modified, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], MESH: Proteome, Paroi, Phenotype, MESH: NADH, GME: GDP-d-mannose-3, Gene Knockdown Techniques, SYSTEMS BIOLOGY, Metabolome, MESH: Oxidoreductases Acting on CH-CH Group Donors, MESH: Fruit, MESH: Metabolome, Metabolic Networks and Pathways, GalLDH: L-Galactono-1, 570, Oxidoreductases Acting on CH-CH Group Donors, Genetically Modified, MESH: Phenotype, Tomato, Systèmes complexes, MESH: Gene Expression Profiling, Tomate, MESH: Analysis of Variance, 580, 4-Lactone Dehydrogenase, Systems biology Ascorbate, Analysis of Variance, Gene Expression Profiling, MESH: Gene Knockdown Techniques, Systems Integration, 5 -epimerase, MESH: Metabolic Networks and Pathways, Fruit, TOMATO, Ascorbate Oxidase, MESH: Systems Integration, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Carbohydrate Epimerases
Complex systems, VTC: Vitamin C, Proteome, [SDV]Life Sciences [q-bio], MESH: Ascorbate Oxidase, MESH: Plants, Ascorbic Acid, AsA: Ascorbic acid, NADPH Oxidoreductases, 630, Biologie intégrative, MDHAR, Solanum lycopersicum, MESH: Lycopersicon esculentum, BIOLOGIE CELLULAIRE, MESH: Ascorbic Acid, AO: Ascorbate oxidase, NADH, NADPH Oxidoreductases, CELL WALL, ASCORBIQUE ACID, Plant Proteins, MESH: Plant Proteins, Monodehydroascorbate reductase Ascorbic acid, MESH: Genomics, Cell wall, Genomics, MESH: Carbohydrate Epimerases, Plants, Genetically Modified, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], MESH: Proteome, Paroi, Phenotype, MESH: NADH, GME: GDP-d-mannose-3, Gene Knockdown Techniques, SYSTEMS BIOLOGY, Metabolome, MESH: Oxidoreductases Acting on CH-CH Group Donors, MESH: Fruit, MESH: Metabolome, Metabolic Networks and Pathways, GalLDH: L-Galactono-1, 570, Oxidoreductases Acting on CH-CH Group Donors, Genetically Modified, MESH: Phenotype, Tomato, Systèmes complexes, MESH: Gene Expression Profiling, Tomate, MESH: Analysis of Variance, 580, 4-Lactone Dehydrogenase, Systems biology Ascorbate, Analysis of Variance, Gene Expression Profiling, MESH: Gene Knockdown Techniques, Systems Integration, 5 -epimerase, MESH: Metabolic Networks and Pathways, Fruit, TOMATO, Ascorbate Oxidase, MESH: Systems Integration, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Carbohydrate Epimerases
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).27 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!