Covid-19 pandemic had an impact on the global economy. Startups played big role in Indonesia’s economy. This study was aimed to know the startups business opportunity during pandemic. This study used online questionnaires to the startups under the coordination of the Ministry of Research and Technology. Questionnaire were processed using pivot tables and analyzed by SWOT. Data showed that 79% startups keep survive despite the pandemic conditions. This study was focus on startups in the field of health/medicine, agriculture/food security, IT, transportation, manufacture, energy. The business sustainability were 95%;86%;76%;75%;71%;43%, respectively. The increase number in turnover were 48%;16%;11%;0%;8%;0%, respectively. The increase number of labor were 29%;6%;7%;0%,4%;0%,respectively. SWOT analysis showed that the startups in the field of health/medicine, agriculture/food security, IT and manufacture have business opportunities to develop amid the pandemic. The result of this study was recommended to arrange policy design for reducing the impact of Covid-19 pandemic on startups business.