Aim. Human sexuality is an important multidimensional subject for psychological research. However, the study of sexuality remains relatively closed for Ukrainian researchers, primarily due to a lack of valid and reliable measures of sexuality in general, and in particular, measures of psychological patterns in human sexuality. Therefore, the aim of the present study was to validate a self-report English-language tool, the Multidimensional Sexual Questionnaire (MSQ), for the Ukrainian language. Methods. A total of 1113 Ukrainian-speaking participants were administered the MSQ-UA. The MSQ-UA factor structure was measured using EFA and CFA procedures. Alpha and Omega estimates of internal consistency were calculated. The test-retest reliability was established in a five-week time period. The correlations of the MSQ-UA subscales with relevant psychopathology, personality measures and control statements were accomplished to prove the construct validity of the questionnaire. Results. Findings indicated that the MSQ-UA is an eleven-dimensional measure, which is almost identical to the original English-language version. Subscales of the MSQ-UA display acceptable excellent psychometric properties of internal consistency (Alpha and Omega within a range of 0.70 to 0.92). The test-retest reliability over the five-week period was appropriate (r=0.68-0.93). The MSQ-UA subscales demonstrated from moderate to high correlations with relevant measures, and the associations were absent or low in cases when criterion measures were irrelevant to the subscale. Conclusion. The MSQ-UA demonstrated appropriate psychometric properties and may be applied to assess a broad range of psychological patterns in sexual relationships in the Ukrainian-speaking population.