Aim. This research aims to reveal the peculiarities of standardization and organizational principles behind the functioning of Orthodox brotherhoods on the territory of Right-Bank Ukraine from the 1850s to the 1900s. Methods. Methodologically, the authors of the work rely on the principles of novelty, objectivity, and historicism and employ general scientific methods (internal criticism of the sources, analysis, synthesis, generalization). Results. The study revealed that during the second half of the 19th century, the activity of Orthodox brotherhoods on the territories controlled by the Russian Empire was regulated by the law “Basic Rules for the Establishment of the Orthodox Church Brotherhoods” which regulated the prioritized tasks, membership and main vectors of their work. At the beginning of the 20th century, some changes occurred in the social-political life of the Russian Empire, which also affected the position of the Orthodox brotherhoods of Right-Bank Ukraine. Conclusion. The church authorities devoted considerable efforts to revitalizing and restoring the activity of the Orthodox brotherhoods at the beginning of the 20th century. In order to find new solutions to the situation, they discussed the further functioning of the fraternities at the congresses in which participated the representatives of the Orthodox brotherhoods of the western provinces of the Russian Empire. Thus, the church management controlled brotherhoods and channelled them into the required course of action.