IMMOBILIZATION OF ACID PHOSPHATASE (TYPE I) FROM WHEAT GERM ON GLUTARALDEHYDE ACTIVATED CHITOSAN BEADS: OPTIMIZATION AND CHARACTERIZATION

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K. Belho ; S.R. Nongpiur ; P.K. Ambasht (2014)
  • Publisher: International Science Press
  • Journal: Journal of Proteins and Proteomics (issn: 0975-8151, eissn: 0975-8151)
  • Subject: Acid phosphatase | wheat germ | immobilization | chitosan | kinetics; reusability | kinetics | reusability | Biochemistry | QD415-436

Acid phosphatase from wheat germ (specific activity 1.327 U/mg protein) was used for immobilization on glutaraldehyde activated chitosan beads. Upon activation of chitosan beads, elongated fibers with pores were observed. The optimum percent immobilization obtaine... View more
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