publication . Article . 2017

Morphological Characterization and Determination of Aflatoxin-Production Potentials of Aspergillus flavus Isolated from Maize and Soil in Kenya

Matome Thathana; Hunja Murage; Akebe Abia; Michael Pillay;
Open Access English
  • Published: 01 Sep 2017 Journal: Agriculture (issn: 2077-0472, Copyright policy)
  • Publisher: MDPI AG
Abstract
This study aimed at morphologically identifying Aspergillus flavus in soil and maize and at determining their aflatoxin-producing potentials. Five hundred and fourteen isolates obtained from maize and soil in Kenya were cultivated on Czapeck Dox Agar, Malt Extract Agar, Sabouraud Dextrose Agar, Potato Dextrose Agar, and Rose-Bengal Chloramphenicol Agar. Isolates were identified using macro-morphological characteristics. Micromorphological characteristics were determined using slide cultures. Aflatoxin production was determined by direct visual determination of the UV fluorescence of colonies on Coconut Agar Medium, Yeast Extract Sucrose agar, and Yeast Extract C...
Subjects
Medical Subject Headings: food and beveragesheterocyclic compoundstechnology, industry, and agriculture
free text keywords: Aflatoxins, Aspergillus flavus, morphological characterization, Thin Layer Chromatography, UV Fluorescence, Agriculture (General), S1-972, Agronomy, Crop, Food science, Biology, Aflatoxin, biology.organism_classification, Potato dextrose agar, Microbiology, Chloramphenicol, medicine.drug, medicine, Agar plate, Agar, food.ingredient, food, Yeast extract
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