publication . Article . 2010

ANÁLISE PROTEÓMICO DE CULTURAS PRELIMINARES DO THYROID

Echeverri, Nancy P; Ortiz, Blanca L; Caminos, Jorge E;
Open Access English
  • Published: 01 Dec 2010 Journal: Revista Colombiana de Química (issn: 0120-2804, eissn: 2357-3791, Copyright policy)
  • Publisher: Universidad Nacional de Colombia
Abstract
En el presente estudio se identificaron proteínas de expresión constitutiva, como vimentina, actina, tubulina, proteína de choque térmico de 60 kDa, peroxirredoxina y la ATP sintasa mitocondrial, en cultivos primarios de tiroides normales y de carcinoma papilar de tiroides. Se establecieron las condiciones de extracción, solubilización, análisis cuantitativo y cualitativo de dichas proteínas, y se buscaron las mejores condiciones de isoelectroenfoque (IEF) en la electroforesis en dos dimensiones (2D). En la extracción y solubilización de las proteínas se evaluó la presencia o ausencia de anfolitos y sales, se obtuvo un mejor resultado empleando en el amortiguado...
Subjects
free text keywords: proteómica, isoelectroenfoque (IEF), electroforesis 2D, proteomics, isoelectrofocousing (IEF), 2D electrophoresis, isoeletroenfoque (IEE), eletroforese 2D, Chemistry, QD1-999
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