publication . Article . 2016

Effect of cooling to different sub-zero temperatures on boar sperm cryosurvival

Angelica Garcia-Olivares; Cesar Garzon-Perez; Oscar Gutiérrez-Pérez; Alfredo Medrano;
Open Access
  • Published: 01 Mar 2016 Journal: Asian Pacific Journal of Reproduction, volume 5, pages 63-66 (issn: 2305-0500, Copyright policy)
  • Publisher: Asian Pacific Journal of Tropical Medicine Press
Abstract
Objective: To compare different cooling temperatures before ice formation on pig sperm quality, before and after cryopreservation. Methods: Semen diluted in BF5 was cooled from 23 °C to 5 °C (1% glycerol, 200 × 106 cells/mL). Sperm were packaged in plastic straws, and maintained at +5 °C per 16 h. 1. Freezing point of diluted spermatozoa was determined by exposing straws to nitrogen vapors. 2. Straws (at +5 °C) were further cooled to −3 °C, −5 °C, and −7 °C, and rewarmed. 3. Straws (at +5 °C) were further cooled to −3 °C and −5 °C, then frozen and stored in liquid nitrogen, and one month later thawed. Progressive motility (PM), viability (Eosin/Nigrosine), plasm...
Persistent Identifiers
Subjects
free text keywords: Spermatozoa, Pig, Cooling, Freeze-thawing, Sperm, Cryopreservation, Liquid nitrogen, Semen, Chemistry, Freezing point, Frost (temperature), Acrosome, Animal science, Glycerol, chemistry.chemical_compound, lcsh:Medicine, lcsh:R
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