Effect of cooling to different sub-zero temperatures on boar sperm cryosurvival

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Angelica Garcia-Olivares; Cesar Garzon-Perez; Oscar Gutierrez-Perez; Alfredo Medrano;

Objective: To compare different cooling temperatures before ice formation on pig sperm quality, before and after cryopreservation. Methods: Semen diluted in BF5 was cooled from 23 °C to 5 °C (1% glycerol, 200 × 106 cells/mL). Sperm were packaged in plastic straws, an... View more
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