publication . Article . 2014

CALLUS INDUCTION FROM 15 CARNATION (DIANTHUS CARYOPHYLLUS L.) CULTIVARS

Jaime A. TEIXEIRA DA SILVA;
Open Access English
  • Published: 01 Dec 2014 Journal: Journal of Plant Development, volume 21, issue 1, pages 15-21 (issn: 2065-3158, eissn: 2066-9917, Copyright policy)
  • Publisher: Alexandru Ioan Cuza University of Iasi
Abstract
Plant growth regulators (PGRs) were used to induce callus in 15 carnation (Dianthus caryophyllus L.; Caryophyllaceae) cultivars: Orange Sherbert, Avalanche, Magenta, La France, Stripe Red, Marie, Concerto PVP, Snap, Lucky Pierot, Cinnamon Tea, White Love, Siberia, Magesta, Spark Bruno, and Honono no Estejo. Seeds were initially sown on autoclaved moistened filter paper and internodes of surface-sterilized seedlings were used as explants. Most callus was induced in the presence of 0.5 mg/L α-naphthaleneacetic acid used together with 1 mg/L 6-benzyladenine or 1 mg/L 2,4-dichlorophenoxyacetic acid on basal Murashige and Skoog medium. Callus is not a desirable metho...
Subjects
free text keywords: carnation, internode, plant growth regulator, lcsh:Plant culture, lcsh:SB1-1110, lcsh:Botany, lcsh:QK1-989
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