publication . Article . 2011

Imaging herpes simplex virus type 1 amplicon vector-mediated gene expression in human glioma spheroids

Christine Kaestle; Alexandra Winkeler; Raphaela Richter; Heinrich Sauer; Jürgen Hescheler; Cornel Fraefel; Maria Wartenberg; Andreas H. Jacobs;
Closed Access English
  • Published: 01 May 2011
  • Publisher: B.C. Decker
  • Country: Switzerland
Abstract
Vectors derived from herpes simplex virus type 1 (HSV-1) have great potential for transducing therapeutic genes into the central nervous system; however, inefficient distribution of vector particles in vivo may limit their therapeutic potential in patients with gliomas. This study was performed to investigate the extent of HSV-1 amplicon vector–mediated gene expression in a three-dimensional glioma model of multicellular spheroids by imaging highly infectious HSV-1 virions expressing green fluorescent protein (HSV-GFP). After infection or microscopy-guided vector injection of glioma spheroids at various spheroid sizes, injection pressures and injection times, th...
Subjects
Medical Subject Headings: embryonic structures
free text keywords: Institute of Virology, 570 Life sciences; biology, Biotechnology, Molecular Medicine, Radiology Nuclear Medicine and imaging, Condensed Matter Physics, Biomedical Engineering, Molecular biology, Genetic enhancement, Transduction (genetics), Green fluorescent protein, Amplicon, Spheroid, Gene expression, Herpes simplex virus, medicine.disease_cause, medicine, Biology, Glioma, medicine.disease, Biology (General), QH301-705.5
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