publication . Article . Other literature type . 2018

Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Larvae of Spodoptera litura Exposed to Azadirachtin Stress Conditions

SETHURAMAN VEERAN;
Open Access English
  • Published: 01 Apr 2018 Journal: Frontiers in Physiology (issn: 1664-042X, Copyright policy)
  • Publisher: Frontiers Media S.A.
Abstract
Azadirachtin is an efficient and broad-spectrum botanical insecticide against more than 150 kinds of agricultural pests with the effects of mortality, antifeedant and growth regulation. Real-time quantitative polymerase chain reaction (RT-qPCR) could be one of the powerful tools to analyze the gene expression level and investigate the mechanism of azadirachtin at transcriptional level, however, the ideal reference genes are needed to normalize the expression profiling of target genes. In this present study, the fragments of eight candidate reference genes were cloned and identified from the pest Spodoptera litura. In addition, the expression stability of these g...
Subjects
free text keywords: Spodoptera litura, azadirachtin, RT-qPCR, reference gene, stability, Physiology, QP1-981, Original Research, Physiology (medical), Internal medicine, medicine.medical_specialty, medicine, Real-time polymerase chain reaction, Biochemistry, Reference genes, Cloning, Endocrinology, Biology, Gene expression profiling, Gene, PEST analysis, biology.organism_classification, chemistry.chemical_compound, chemistry
Related Organizations
52 references, page 1 of 4

Andersen C. L.Jensen J. L.Ørntoft T. F. (2004). Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 5245–5250. 10.1158/0008-5472.CAN-04-0496 15289330 [PubMed] [DOI]

Anuradha A.Annadurai R. S. (2008). Biochemical and molecular evidence of azadirachtin binding to insect actins. Curr. Sci. India 95, 1588–1593.

Anuradha A.Annadurai R. S.Shashidhara L. S. (2007). Actin cytoskeleton as a putative target of the neem limonoid azadirachtin A. Insect Biochem. Molec. 37, 627–634. 10.1016/j.ibmb.2007.03.009 17517339 [OpenAIRE] [PubMed] [DOI]

Arya S. K.Jain G.Upadhyay S. K.Sarita S. H.Dixit S.Verma P. C. (2017). Reference genes validation in Phenacoccus solenopsis under various biotic and abiotic stress conditions. Sci. Rep. 7:13520. 10.1038/s41598-017-13925-9 29051594 [OpenAIRE] [PubMed] [DOI]

Bano A.Muqarab R. (2017). Plant defence induced by PGPR against Spodoptera litura in tomato (Solanum lycopersicum L.). Plant Biol. 19, 406–412. 10.1111/plb.12535 28004873 [OpenAIRE] [PubMed] [DOI]

Chandra G. S.Asokan R.Manamohan M.Kumar N. K. K.Sita T. (2017). Evaluation of reference genes for quantitative real-time PCR normalization in cotton bollworm Helicoverpa armigera. Mol. Biol. 48, 813–822. 10.1134/S0026893314060156 [DOI]

Chen H.Yang Z.Hu Y.Tan J.Jia J.Xu H. (2016). Reference genes selection for quantitative gene expression studies in Pinus massoniana L. Trees-Struct. Func. 30, 685–696. 10.1007/s00468-015-1311-3 [OpenAIRE] [DOI]

Cheng T.Wu J.Wu Y.Chilukuri R. V.Huang L.Yamamoto K.. (2017). Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest. Nat. Ecol. Evol.1, 1747–1756. 10.1038/s41559-017-0314-4 28963452 [OpenAIRE] [PubMed] [DOI]

Feng B.Zheng K.Li C.Guo Q.Du Y. (2017). A cytochrome P450 gene plays a role in the recognition of sex pheromones in the tobacco cutworm, Spodoptera litura. Insect Mol. Biol. 26, 369–382. 10.1111/imb.12307 28390075 [OpenAIRE] [PubMed] [DOI]

Fu W.Xie W.Zhang Z.Wang S. L.Wu Q. J.Liu Y.. (2013). Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae). Int. J. Biol. Sci. 9, 792–802. 10.7150/ijbs.5862 23983612 [OpenAIRE] [PubMed] [DOI]

Gong L.Wang H. D.Qi J. W.Han L. Z.Hu M. Y.Jurat-Fuentes J. L. (2015). Homologs to Cry toxin receptor genes in a de novo transcriptome and their altered expression in resistant Spodoptera litura larvae. J. Invertebr. Pathol. 129, 1–6. 10.1016/j.jip.2015.05.008 25981133 [OpenAIRE] [PubMed] [DOI]

Guo H.Jiang L.Xia Q. Y. (2016). Selection of reference genes for analysis of stress-responsive genes after challenge with viruses and temperature changes in the silkworm Bombyx mori. Mol. Genet. Genomics 291, 999–1004. 10.1007/s00438-015-1125-4 26437927 [OpenAIRE] [PubMed] [DOI]

Huang X. Y.Li O. W.Xu H. H. (2010). Induction of programmed death and cytoskeletal damage on Trichoplusia ni BTI-Tn-5B1-4 cells by azadirachtin. Pestic. Biochem. Phys. 98, 289–295. 10.1016/j.pestbp.2010.06.020 [OpenAIRE] [DOI]

Ibanez F.Tamborindeguy T. (2016). Selection of reference genes for expression analysis in the potato psyllid, Bactericera cockerelli. Insect Mol. Biol. 25, 227–238. 10.1111/imb.12219 26936438 [OpenAIRE] [PubMed] [DOI]

Jank T.Belyi Y.Wirth C.Rospert S.Hu Z.Dengjel J.. (2017). Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor1A. J. Biol. Chem.292, 16014–16023. 10.1074/jbc.M117.801035 28801462 [OpenAIRE] [PubMed] [DOI]

52 references, page 1 of 4
Abstract
Azadirachtin is an efficient and broad-spectrum botanical insecticide against more than 150 kinds of agricultural pests with the effects of mortality, antifeedant and growth regulation. Real-time quantitative polymerase chain reaction (RT-qPCR) could be one of the powerful tools to analyze the gene expression level and investigate the mechanism of azadirachtin at transcriptional level, however, the ideal reference genes are needed to normalize the expression profiling of target genes. In this present study, the fragments of eight candidate reference genes were cloned and identified from the pest Spodoptera litura. In addition, the expression stability of these g...
Subjects
free text keywords: Spodoptera litura, azadirachtin, RT-qPCR, reference gene, stability, Physiology, QP1-981, Original Research, Physiology (medical), Internal medicine, medicine.medical_specialty, medicine, Real-time polymerase chain reaction, Biochemistry, Reference genes, Cloning, Endocrinology, Biology, Gene expression profiling, Gene, PEST analysis, biology.organism_classification, chemistry.chemical_compound, chemistry
Related Organizations
52 references, page 1 of 4

Andersen C. L.Jensen J. L.Ørntoft T. F. (2004). Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 5245–5250. 10.1158/0008-5472.CAN-04-0496 15289330 [PubMed] [DOI]

Anuradha A.Annadurai R. S. (2008). Biochemical and molecular evidence of azadirachtin binding to insect actins. Curr. Sci. India 95, 1588–1593.

Anuradha A.Annadurai R. S.Shashidhara L. S. (2007). Actin cytoskeleton as a putative target of the neem limonoid azadirachtin A. Insect Biochem. Molec. 37, 627–634. 10.1016/j.ibmb.2007.03.009 17517339 [OpenAIRE] [PubMed] [DOI]

Arya S. K.Jain G.Upadhyay S. K.Sarita S. H.Dixit S.Verma P. C. (2017). Reference genes validation in Phenacoccus solenopsis under various biotic and abiotic stress conditions. Sci. Rep. 7:13520. 10.1038/s41598-017-13925-9 29051594 [OpenAIRE] [PubMed] [DOI]

Bano A.Muqarab R. (2017). Plant defence induced by PGPR against Spodoptera litura in tomato (Solanum lycopersicum L.). Plant Biol. 19, 406–412. 10.1111/plb.12535 28004873 [OpenAIRE] [PubMed] [DOI]

Chandra G. S.Asokan R.Manamohan M.Kumar N. K. K.Sita T. (2017). Evaluation of reference genes for quantitative real-time PCR normalization in cotton bollworm Helicoverpa armigera. Mol. Biol. 48, 813–822. 10.1134/S0026893314060156 [DOI]

Chen H.Yang Z.Hu Y.Tan J.Jia J.Xu H. (2016). Reference genes selection for quantitative gene expression studies in Pinus massoniana L. Trees-Struct. Func. 30, 685–696. 10.1007/s00468-015-1311-3 [OpenAIRE] [DOI]

Cheng T.Wu J.Wu Y.Chilukuri R. V.Huang L.Yamamoto K.. (2017). Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest. Nat. Ecol. Evol.1, 1747–1756. 10.1038/s41559-017-0314-4 28963452 [OpenAIRE] [PubMed] [DOI]

Feng B.Zheng K.Li C.Guo Q.Du Y. (2017). A cytochrome P450 gene plays a role in the recognition of sex pheromones in the tobacco cutworm, Spodoptera litura. Insect Mol. Biol. 26, 369–382. 10.1111/imb.12307 28390075 [OpenAIRE] [PubMed] [DOI]

Fu W.Xie W.Zhang Z.Wang S. L.Wu Q. J.Liu Y.. (2013). Exploring valid reference genes for quantitative real-time PCR analysis in Plutella xylostella (Lepidoptera: Plutellidae). Int. J. Biol. Sci. 9, 792–802. 10.7150/ijbs.5862 23983612 [OpenAIRE] [PubMed] [DOI]

Gong L.Wang H. D.Qi J. W.Han L. Z.Hu M. Y.Jurat-Fuentes J. L. (2015). Homologs to Cry toxin receptor genes in a de novo transcriptome and their altered expression in resistant Spodoptera litura larvae. J. Invertebr. Pathol. 129, 1–6. 10.1016/j.jip.2015.05.008 25981133 [OpenAIRE] [PubMed] [DOI]

Guo H.Jiang L.Xia Q. Y. (2016). Selection of reference genes for analysis of stress-responsive genes after challenge with viruses and temperature changes in the silkworm Bombyx mori. Mol. Genet. Genomics 291, 999–1004. 10.1007/s00438-015-1125-4 26437927 [OpenAIRE] [PubMed] [DOI]

Huang X. Y.Li O. W.Xu H. H. (2010). Induction of programmed death and cytoskeletal damage on Trichoplusia ni BTI-Tn-5B1-4 cells by azadirachtin. Pestic. Biochem. Phys. 98, 289–295. 10.1016/j.pestbp.2010.06.020 [OpenAIRE] [DOI]

Ibanez F.Tamborindeguy T. (2016). Selection of reference genes for expression analysis in the potato psyllid, Bactericera cockerelli. Insect Mol. Biol. 25, 227–238. 10.1111/imb.12219 26936438 [OpenAIRE] [PubMed] [DOI]

Jank T.Belyi Y.Wirth C.Rospert S.Hu Z.Dengjel J.. (2017). Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor1A. J. Biol. Chem.292, 16014–16023. 10.1074/jbc.M117.801035 28801462 [OpenAIRE] [PubMed] [DOI]

52 references, page 1 of 4
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publication . Article . Other literature type . 2018

Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Larvae of Spodoptera litura Exposed to Azadirachtin Stress Conditions

SETHURAMAN VEERAN;