publication . Article . 2014

Determine the prevalence of Brucella spp. and Leptospira spp. in blood samples by multiplex polymerase chain reaction collected from cattle, sheep and goats in herds located in provinces of Iran

Faham Khamesipour; Shahin Nejat Dehkordi; Taghi Taktaz Hafshejani; Elahe Tajbakhsh; Shahrzad Azizi;
Open Access English
  • Published: 01 May 2014 Journal: Veterinary Science Development (issn: 2038-9698, eissn: 2038-9701, Copyright policy)
  • Publisher: PAGEPress Publications
Abstract
Leptospirosis and brucellosis are common zoonosis that affect many species of mammals mostly causing economical losses. Further, very important fact is huge danger for human and animal health around the world. The purpose of the study is to determine the prevalence of <em>Brucella</em> spp. and <em>Leptospira</em> spp. using multiplex polymerase chain reaction (mPCR) method, in blood samples collected from cattle, sheep and goats. In this study, a total number of 250 blood samples (5 cc of blood with ethilen diamin tetra asetic acid) were collected randomly from 100 cattle, 80 sheep and 70 goats located on 6 herds in Chaharmahal Va Bakhtiari and Esfahan province...
Subjects
mesheuropmc: bacterial infections and mycoses
free text keywords: QL1-991, Brucella spp., Leptospira spp., Iran, multiplex polymerase chain reaction, ruminant, blood, Zoology
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