publication . Article . 2017

Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates

Jue-Liang Hsu; Nur Maulida Safitri; Endang Yuli Herawati;
Open Access English
  • Published: 01 Aug 2017 Journal: Research Journal of Life Science (issn: 2355-9926, Copyright policy)
  • Publisher: University of Brawijaya
Abstract
The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae . The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.
Subjects
free text keywords: Antioxidant, Cyanobacteria, DPPH, LC-MS, RP-HPLC, Agriculture (General), S1-972, Medicine (General), R5-920, Thermolysin, Enzyme, chemistry.chemical_classification, chemistry, Proteolytic enzymes, Hydrolysate, Chromatography, chemistry.chemical_compound, medicine.medical_treatment, medicine, Peptide, Fractionation
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