Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates
Nur Maulida Safitri
Endang Yuli Herawati
Jue Liang Hsu
- Publisher: University of Brawijaya
Research Journal of Life Science
Antioxidant | Cyanobacteria | DPPH | LC-MS | RP-HPLC | Agriculture (General) | S1-972 | Medicine (General) | R5-920
The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.