EVA GREEN REAL-TIME PCR USED TO DETECT CELERY AS AN ALLERGEN IN FOOD

Article English OPEN
Ondrej Škultéty ; Andrea Jurčáková (2011)
  • Publisher: HACCP Consulting
  • Journal: Potravinarstvo (issn: 1338-0230, eissn: 1337-0960)
  • Related identifiers: doi: 10.5219/138
  • Subject: apium graveolens | food analysis | detection limit | polymerase chain reaction | Nutrition. Foods and food supply | TX341-641

<span style="font-size: small;"><div style="mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 175.9pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: auto; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 52.45pt; mso-element-top: 55.2pt; mso-height-rule: exactly;"><table style="height: 243px;" border="0" cellspacing="0" cellpadding="0" width="664"><tbody><tr><td style="padding-top: 3.1pt; padding-right: 0cm; padding-bottom: 3.1pt; padding-left: 0cm;" height="243" align="left" valign="top"><div style="mso-element: para-border-div; border: none; border-bottom: solid windowtext 2.25pt; padding: 0cm 0cm 1.0pt 0cm;"><p class="MsoNormal" style="border: none; mso-border-bottom-alt: solid windowtext 2.25pt; padding: 0cm; mso-padding-alt: 0cm 0cm 1.0pt 0cm; mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 175.9pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: auto; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 52.45pt; mso-element-top: 55.2pt; mso-height-rule: exactly;"><span style="font-size: 10.0pt; mso-ansi-language: EN-GB;" lang="EN-GB">EvaGreen&reg;<span style="mso-spacerun: yes;">&nbsp; </span>Real-Time PCR method has been used for celery(<em style="mso-bidi-font-style: normal;">Apium graveolens</em>) allergen detection. A primer designed in mannitol dehydrogenase gene region has been used for specific celery identification in sample. The results show possibility to create calibration curve using artificially adulterated samples. The increasing variability between parallel calibration of celery samples has been observed from 0.1 % to 100%. Detection limit has been set to value 0.1% in celery representing 1000 ppm. Fluorescent signal has been presented even in samples with lower percentage addition of celery but these samples have been excluded according to unspecific melting curve.<br /><span style="color: #111111; font-size: 9px;"><br /><strong>doi:10.5219/138</strong></span></span></p></div></td></tr></tbody></table></div></span>
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