publication . Article . 2010

c-DNA of HIV-1 detection on spot of Buffy-Coat of leukocytes (DBCS)

Marco Rossi de Gasperis; Maria Daniela Caione; Carlo Concato; Ersilia Fiscarelli; Nicola Di Pietro; Vittorio Salotti; Lorenza Putignani; Donato Menichella; Francesco Callea;
Open Access
  • Published: 01 Mar 2010 Journal: Microbiologia Medica, volume 25 (issn: 2280-6423, Copyright policy)
  • Publisher: PAGEPress Publications
Introduction:The elective way for the diagnosis of HIV-1-infection in the window period and in children under the age of 16-18 months is to search virus integrated in leukocytes. Aim of the study was to assess the sensitivity and specificity of extraction from Buffy-Dried Coat Spot (DBCS) in leukocyte to detect c-DNA with nested-PCR in HIV-1-infected individuals compared to Dried Blood Spot (DBS) both extracted by automated instrument EZ1 (QIAGEN, Hilden, Germany). Both DBCS and both DBS were compared with those tests from whole blood by conventional DNA-extraction Methods: Five ml of whole blood from 50 HIV-infected individuals were collected. 40 μl of each sam...
free text keywords: HIV-1 (Human Immunodeficiency Virus Type 1), DBS (Dried Blood Spot), DBCS (Dried Buffy Coat Spot), Microbiology, QR1-502
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