publication . Article . 2017

RNA isolation from bloodstains collected on FTA cards – application in clinical and forensic genetics

Katarzyna Skonieczna; Jan Styczyński; Anna Krenska; Mariusz Wysocki; Aneta Jakubowska; Tomasz Grzybowski;
Open Access English
  • Published: 01 Jun 2017 Journal: Archives of Forensic Medicine and Criminology, volume 66, issue 4, pages 244-254 (issn: 0324-8267, eissn: 1689-1716, Copyright policy)
  • Publisher: Termedia Publishing House
Abstract
Aim of the study : In recent years, RNA analysis has been increasingly used in clinical and forensic genetics. Nevertheless, a major limitation of RNA-based applications is very low RNA stability in biological material, due to the RNAse activity. This highlights the need for improving the methods of RNA collection and storage. Technological approaches such as FTA Classic Cards (Whatman) could provide a solution for the problem of RNA degradation. However, different methods of RNA isolation from FTA cards could have diverse effects on RNA quantity and quality. The purpose of this research was to analyze the utility of three different methods of RNA isolation from...
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Subjects
free text keywords: bloodstains, RNA, RNA degradation, gene expression, FTA cards, lcsh:Medicine, lcsh:R, lcsh:Social pathology. Social and public welfare. Criminology, lcsh:HV1-9960, Trizol, Computational biology, Virology, microRNA, Reverse transcriptase, RNA Stability, Biology, RNA extraction, Forensic genetics
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