publication . Article . 2013

Development of EST-derived markers in Dendrobium from EST of related taxa

Narisa Juejun; Chataporn Chunwongse; Julapark Chunwongse;
Open Access English
  • Published: 01 Apr 2013 Journal: Songklanakarin Journal of Science and Technology (SJST) (issn: 0125-3395, Copyright policy)
  • Publisher: Prince of Songkla University
Public databases are useful for molecular marker development. The major aim of this study was to develop expressedsequence tag (EST)-derived markers in Dendrobium from available ESTs of Phalaenopsis and Dendrobium. A total of 6063sequences were screened for simple sequence repeats (SSRs) and introns. Primers flanking these regions were generated andtested on genomic DNAs of Phalaenopsis and Dendrobium. Twenty-three percent of amplifiable Phalaenopsis EST-derivedmarkers were cross-genera transferable to Dendrobium. Forty-one markers from both Phalaenopsis and Dendrobium thatamplified in Dendrobium were assessed on six commercial cultivars and six wild accessions....
Medical Subject Headings: food and beverages
free text keywords: EST-derived marker, EST data analysis, Dendrobium, Phalaenopsis, synteny, Technology, T, Technology (General), T1-995, Science, Q, Science (General), Q1-390
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