publication . Article . 2013

Variabilidad genética de aislamientos colombianos del Potato mop-top virus (PMTV).

Inés Osorio-Giraldo; Pablo Gutiérrez-Sánchez; Mauricio Marín-Montoya;
Open Access
  • Published: 09 May 2013 Journal: Agronomía Mesoamericana, volume 24, page 1 (issn: 1021-7444, eissn: 2215-3608, Copyright policy)
  • Publisher: Universidad de Costa Rica
Abstract
The objective of this work was to study the level of variation of PMTV isolates from Colombian potato crops through 2010 and 2011. Genes encoding for the viral coat protein (CP, ARN 2) and the Triple Gene Block (TGB2, ARN 3) were sequenced from PMTV isolates obtained in the provinces of Antioquia, Boyacá, Cundinamarca y Nariño. Additionally, RNA 2 and RNA 3 from two isolates were almost sequenced to completion (>83%). Phylogenetic analysis based on the CP sequence revealed the presences of two clades. The first clade included PMTV reference strains from all over the world and 19 Colombian isolates used in this study. The second class grouped only Colombian is...
Subjects
free text keywords: RT-PCR, sequencing, Solanum tuberosum., secuenciación, Potato mop-top virus, biology.organism_classification, biology, Pomovirus, Clade, DNA sequencing, Plant virus, Botany, Phylogenetic tree, Phylogenetics, Genetics, Whole genome sequencing, Agriculture, S
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