publication . Other literature type . Article . 2015

Molecular characterization of Giardia intestinalis assemblage E from goat kids in Bangladesh

Mohammad Alamgir Hossain; Sohana Akter Mina; Lolo Wal Marzan; Mohammad Asif Imran Khan Emon; Rasel Das; AMAM Zonaed Siddiki;
  • Published: 01 May 2015
  • Publisher: Elsevier BV
Abstract
Abstract Objective To molecularly characterize Giardia in goat kids to elucidate the possible zoonotic hazards in Bangladesh and find out the role of Giardia protozoan parasite as a source of human infection. Methods Fecal samples of 100 goat kids were genotyped by nested PCR amplification of β-giardin gene fragment followed by sequencing and analysis. Results The total prevalence of Giardia in goat kids was 3% (3/100) and the infection is more widespread in younger ( P = 0.36), Black Bengal breed ( P = 0.81) and female goat kids ( P = 0.58). Further analysis of β - giardin gene locus has shown that the gene clustered in assemblage E rather than assemblages A an...
Subjects
Medical Subject Headings: human activities
free text keywords: Breed, Virology, Genetic diversity, Giardia, biology.organism_classification, biology, Feces, Veterinary medicine, Nested polymerase chain reaction, Parasite hosting, Locus (genetics), Genotype, Microbiology, Giardiasis, Nested PCR, Phylogenetic tree, Sequencing, Medicine, R, Arctic medicine. Tropical medicine, RC955-962
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