publication . Article . 2017

NS1 of H7N9 Influenza A Virus Induces NO-Mediated Cellular Senescence in Neuro2a Cells

Kangsheng Li; Jieling Chen; Gefei Wang; Yongming Du; Rui Li; Huali Zheng; Yinxia Yan;
Open Access English
  • Published: 01 Oct 2017 Journal: Cellular Physiology and Biochemistry (issn: 1015-8987, eissn: 1421-9778, Copyright policy)
  • Publisher: Cell Physiol Biochem Press GmbH & Co KG
Abstract
<jats:p>Background/Aims: The novel avian H7N9 influenza A virus has been detected in brain tissues and associated with central nervous system (CNS) symptoms in infected human and mice. Roles of its virulence factor, NS1 protein in influenza virus infected neuron has yet to be explored. Methods: Nitric oxide (NO) release and inducible nitric oxide synthase (iNOS) expression in H7N9/NS1-expressed Neuro2a cells were detected by Griess test and western blotting. Cell proliferation rate of H7N9/NS1-expressing cells was recorded by Cell Counting Kit-8. Effects of H7N9/NS1 on cellular senescence were investigated by senescence-associated β-galactosidase (SA-β-gal) stai...
Subjects
Medical Subject Headings: viruses
free text keywords: H7N9 influenza virus, NS1, Nitric oxide, Cellular senescence, p38 MAPK, Physiology, QP1-981, Biochemistry, QD415-436, Virus, p38 mitogen-activated protein kinases, chemistry.chemical_compound, chemistry, Virology, Influenza A virus, medicine.disease_cause, medicine, Cell growth, Blot, Molecular biology, Nitric oxide synthase, biology.protein, biology, Neuron, medicine.anatomical_structure
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