publication . Article . 2009

Establishment of a continuous culture system for Entamoeba muris and analysis of the small subunit rRNA gene

Kobayashi S.; Suzuki J.; Takeuchi T.;
Open Access English
  • Published: 01 Jun 2009 Journal: Parasite (issn: 1252-607X, eissn: 1776-1042, Copyright policy)
  • Publisher: EDP Sciences
Abstract
We established a culture system for Entamoeba muris (MG-EM-01 strain isolated from a Mongolian gerbil) using a modified Balamuth’s egg yolk infusion medium supplemented with 4% adult bovine serum and Bacteroides fragilis cocultured with Escherichia coli. Further, encystation was observed in the culture medium. The morphological characteristics of E. muris are similar to those of Entamoeba coli (E. coli); moreover, the malic isoenzyme electrophoretic band, which shows species-specific electrophoretic mobility, of E. muris had almost the same mobility as that observed with the malic isoenzyme electrophorectic band of E. coli (UZG-EC-01 strain isolated from a goril...
Subjects
mesheuropmc: parasitic diseases
free text keywords: phylogenetic analysis, dixenic culture, RC109-216, SSU-rRNA gene, Entamoeba muris, Balamuth’s egg yolk infusion medium, Infectious and parasitic diseases, Entamoeba coli
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