publication . Article . Other literature type . 2018

Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study

Angela Pires Brandão; Nora Morcillo; Anandi Martin; Niaina Rakotosamimanana; Juan Carlos Palomino; M C Cergole Novella; Marie Sylvianne Rabodoarivelo; Voahangy Rasolofo; Belén Imperiale; A G C Bombonatte;
Open Access English
  • Published: 01 Jan 2018
  • Publisher: Medknow Publications
Abstract
Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study. Molecular detection of RIF and INH resistance was performed directly from smear-positive TB sputa spotted on a slide, FTA card, GenoCard, and ethanol using the Genotype MTBDRplus assay. The perfor...
Subjects
free text keywords: FILTER CARDS, MOLECULAR DIAGNOSIS, MULTIDRUG-RESISTANT DETECTION, MYCOBACTERIUM TUBERCULOSIS, SMEAR SLIDES, Bioquímica y Biología Molecular, Ciencias Biológicas, CIENCIAS NATURALES Y EXACTAS, Multi-drug resistance, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Antitubercular Agents, DNA, Bacterial, Drug Resistance, Multiple, Bacterial, Genotype, Genotyping Techniques, Humans, Isoniazid, Microbial Sensitivity Tests, Molecular Diagnostic Techniques, Rifampin, Sensitivity and Specificity, Tuberculosis, Multidrug-Resistant, Microbiology (medical), Infectious Diseases, QR1-502, Tuberculosis, medicine.disease, medicine, Microbiology, Drug resistance, Rifampicin, medicine.drug, DNA extraction, Sputum, medicine.symptom, business.industry, business, biology.organism_classification, biology, Multiple drug resistance
16 references, page 1 of 2

1Mycobacteria Unit, Institut Pasteur de Madagascar, Antananarivo, 2Ecole Doctorale Sciences de la Vie et de l'Environnement, Université d'Antananarivo, Antananarivo, Madagascar, 3Instituto Adolfo Lutz Núcleo de Tuberculose e Micobacteriosis Sao Paulo, Sao Paulo, 4Fundação Oswaldo Cruz, Rio de Janeiro, 5Instituto Adolfo Lutz, CRL‑Santo Andre, 6Instituto Adolfo Lutz, CRL‑Santos, Brazil, 7Institute of Experimental Medicine‑CONICET, National Academy of Medicine, Buenos Aires, 8Dr. Cetrangolo Hospital, Reference Laboratory of Tuberculosis Control Program, Buenos Aires, Argentina, 9Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, 10Université catholique de Louvain (UCL), Institute of Experimental and Clinical Research (IREC), Laboratory of Medical Microbiology (MBLG), Brussels, Belgium 1.

Zignol M, van Gemert W, Falzon D, Sismanidis C, Glaziou P, Floyd K, et al. Surveillance of anti‑tuberculosis drug resistance in the world: An updated analysis, 2007‑2010. Bull World Health Organ 2012;90:111‑9D.

World Health Organisation. Global Tuberculosis Report 2017. Geneva; 2017. Available from: http://www.who.int/tb/publications/global_ report/en/. [Last accessed on 2017 Nov 03].

World Health Organisation. International Standards for Tuberculosis Care 2006. Geneva; 2006. Available from: http://www.who.int/tb/ publications/2006/istc/en/. [Last accessed on 2017 Jun 30].

Diagnostic standards and classification of tuberculosis in adults and children. This official statement of the American thoracic society and the centers for disease control and prevention was adopted by the ATS board of directors, July 1999. This statement was endorsed by the council of the infectious disease society of America, September 1999. Am J Respir Crit Care Med 2000;161:1376‑95.

Domingo S. DOTS plus strategy in resource‑poor countries. Int J Tuberc Lung Dis 1999;3:843‑6.

Palomino JC, Vandamme P, Martin A. Classical and new assays for detecting drug resistance in tuberculosis. Biomark Med 2014;8:1105‑14.

Espinal MA, Laszlo A, Simonsen L, Boulahbal F, Kim SJ, Reniero A, et al. Global trends in resistance to antituberculosis drugs. World Health Organization‑International Union against Tuberculosis and Lung Disease Working Group on Anti‑Tuberculosis Drug Resistance Surveillance. N Engl J Med 2001;344:1294‑303. [OpenAIRE]

8. World Health Organization. Guidelines for Surveillance of Drug Resistance. 5th ed. Geneva; 2015. Available from: h ttp://www.who.int/ tb/publications/2015/drs_guidelines/en/. [Last accessed on 2017 Jun 30].

9. Rabodoarivelo MS, Imperiale B, Andrianiavomikotroka R, Brandao A, Kumar P, Singh S, et al. Performance of four transport and storage systems for molecular detection of multidrug‑resistant tuberculosis. PLoS One 2015;10:e0139382.

10. Scott LE, Gous N, Cunningham BE, Kana BD, Perovic O, Erasmus L, et al. Dried culture spots for Xpert MTB/RIF external quality assessment: Results of a phase 1 pilot study in South Africa. J Clin Microbiol 2011;49:4356‑60.

11. Gous N, Cunningham B, Kana B, Stevens W, Scott LE. Performance monitoring of Mycobacterium tuberculosis dried culture spots for use with the geneXpert system within a national program in South Africa. J Clin Microbiol 2013;51:4018‑21.

12. Gous N, Isherwood LE, David A, Stevens W, Scott LE. A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material. J Clin Microbiol 2015;53:1365‑7.

13. Gomgnimbou MK, Refrégier G, Diagbouga SP, Adama S, Kaboré A, Ouiminga A, et al. Spoligotyping of Mycobacterium africanum, Burkina Faso. Emerg Infect Dis 2012;18:117‑9. [OpenAIRE]

14. de Almeida IN, Da Silva Carvalho W, Rossetti ML, Costa ER, De Miranda SS. Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR‑IS6110: Preliminary study. BMC Res Notes 2013;6:561.

16 references, page 1 of 2
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