publication . Article . 2015

Dickkopf1 Up-Regulation Induced by a High Concentration of Dexamethasone Promotes Rat Tendon Stem Cells to Differentiate Into Adipocytes.

Wan Chen; Hong Tang; Xiangzhou Liu; Mei Zhou; Jiqiang Zhang; Kanglai Tang;
Open Access English
  • Published: 01 Nov 2015 Journal: Cellular Physiology and Biochemistry (issn: 1015-8987, eissn: 1421-9778, Copyright policy)
  • Publisher: Cell Physiol Biochem Press GmbH & Co KG
Abstract
Background/Aims: Dexamethasone (Dex)-induced spontaneous tendon rupture and decreased self-repair capability is very common in clinical practice. The metaplasia of adipose tissue in the ruptured tendon indicates that Dex may induce tendon stem cells (TSCs) to differentiate into adipocytes, but the mechanism remains unclear. In the present study, we used in vitro methods to investigate the effects of Dex on rat TSC differentiation and the molecular mechanisms underlying this process. Methods: First, we used qPCR and Western blotting to detect the expression of the adipogenic differentiation markers aP2 and C/EBPα after treating the TSCs with Dex. Oil red staining...
Subjects
Medical Subject Headings: hormones, hormone substitutes, and hormone antagonists
free text keywords: Tendon stem cells, Adipocytes, Dickkopf1, GSK-3β, β-catenin, Dexamethasone, Physiology, QP1-981, Biochemistry, QD415-436, Stem cell, Wnt signaling pathway, Molecular biology, Ccaat-enhancer-binding proteins, Adipogenesis, DKK1, Biology, Cellular differentiation, GSK-3, Beta-catenin, biology.protein
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publication . Article . 2015

Dickkopf1 Up-Regulation Induced by a High Concentration of Dexamethasone Promotes Rat Tendon Stem Cells to Differentiate Into Adipocytes.

Wan Chen; Hong Tang; Xiangzhou Liu; Mei Zhou; Jiqiang Zhang; Kanglai Tang;