Effects of environmental preconditioning, donor tissue and isolation conditions on tomato (Lycopersicon esculentum Mill.) protoplast yield

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Elżbieta Kuźniak; Marzena Wielanek; Urszula Małolepsza; Henryk Urbaniak;
  • Publisher: Polish Botanical Society
  • Journal: Acta Agrobotanica (issn: 2300-357X)
  • Related identifiers: doi: 10.5586/aa.1995.022
  • Subject: S1-972 | Agriculture (General)
    mesheuropmc: food and beverages | biochemical phenomena, metabolism, and nutrition | bacteria | fungi

The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x 107/g FW was obtained from l... View more
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