publication . Article . 2014

Deteksi dan Identifikasi Potyvirus pada Ubi Jalar di Tana Toraja, Sulawesi Selatan

Laras Anjarsari; Gede Suastika; Tri Asmira Damayanti;
Open Access Indonesian
  • Published: 01 Aug 2014 Journal: Jurnal Fitopatologi Indonesia (issn: 0215-7950, eissn: 2339-2479, Copyright policy)
  • Publisher: Perhimpunan Fitopatologi Indonesia
Abstract
<p class="Pa1"><span class="A4"><span>Typical viral symptoms including chlorotic and uneven interveinal yellowing on leaves without leaf malformation was observed on sweet potato field in Tana Toraja, South Sulawesi. To identify the causal of the disease, reverse transcription-polymerase chain reaction (RT-PCR) and DNA sequencing were carried out to detect the virus from infected plants. RT-PCR using universal primer for C1 gene of </span></span><span class="A4"><span>Potyvirus </span></span><span class="A4"><span>was successfully amplified approximately 683bp DNA fragment. The nucleotide sequences of this C1 gene fragment showed 98% homology to </span></span><s...
Subjects
Medical Subject Headings: food and beverages
free text keywords: chlorosis, leaf spot, RT-PCR, Sweet potato virus G, Botany, QK1-989
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