publication . Article . Other literature type . 2019

СИСТЕМА РЕГЕНЕРАЦИИ САЛАТА ( LACTUCA SATIVA L.) ЧЕРЕЗ КУЛЬТУРУ IN VITRO

O. V. Romanova; A. V. Soldatenko; V. S. Romanov;
Open Access English
  • Published: 14 Jun 2019 Journal: Ovoŝi Rossii (issn: 2072-9146, Copyright policy)
  • Publisher: Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"
Abstract
<jats:p>The article is devoted to the problem of obtaining regenerative plant varieties of lettuce (<jats:underline>Lactuca sativa</jats:underline> L.) Bouquet, Emerald, Chameleon (FSBSI Federal Scientific Vegetable Center). Salad culture is insensitive to <jats:underline>in vitro</jats:underline> conditions and requires the development of alternative regeneration conditions. Seeds were surface sterilized in 96% ethanol (30 s), then 15 minutes in 50% aqueous solution of "Whiteness" with the addition of Twin-20 (1 drop per 100 ml), washed three times in sterile distilled water. Then lettuce seeds were placed in Petri dishes on Gamborg B5 medium (2% sucrose, 3,0 g...
Subjects
free text keywords: lettuce, <i>lactuca sativa</i> l., biotechnology, <i>in vitro</i>, clonal reproduction, regenerative plants, Agriculture, S, Sucrose, chemistry.chemical_compound, chemistry, Agar, food.ingredient, food, Distilled water, Murashige and Skoog medium, Shoot, Petri dish, law.invention, law, Explant culture, Lactuca, biology.organism_classification, biology, Horticulture
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publication . Article . Other literature type . 2019

СИСТЕМА РЕГЕНЕРАЦИИ САЛАТА ( LACTUCA SATIVA L.) ЧЕРЕЗ КУЛЬТУРУ IN VITRO

O. V. Romanova; A. V. Soldatenko; V. S. Romanov;