publication . Article . 2016

Effectiveness of mesenchymal stems cells cultured by hanging drop vs. conventional culturing on the repair of hypoxic-ischemic-damaged mouse brains, measured by stemness gene expression

Laijun Song; Hui Zhang; Yongli Lou; Dewei Guo;
Open Access
  • Published: 01 Jan 2016 Journal: Open Life Sciences, volume 11, pages 519-523 (eissn: 2391-5412, Copyright policy)
  • Publisher: Walter de Gruyter GmbH
Abstract
<jats:title>Abstract</jats:title><jats:p>In this study, we investigated the therapeutic effects of Human Mesenchymal Stem Cells (hMSCs) cultured by hanging drop and conventional culturing methods on cerebellar repair in hypoxic-ischemic (HI) brain injured mice. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to analyze the expression levels of three stemness genes, Oct4, Sox2 and Nanog, and the migration related gene CXCR4. MSC prepared by hanging drop or conventional techniques were administered intranasally to nine day old mice, and analyzed by MRI at day 28. Results indicate that the MSCs, especially the hanging drop ...
Subjects
free text keywords: MSC, stemness, brain injury, Biology (General), QH301-705.5, Mesenchymal stem cell, Cell biology, Biology, Gene expression, Molecular biology, Hypoxic ischemic
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