publication . Article . 2015

Studies on antigenic cross-reactivity of Trichuris ovis with host mucosal antigens in goat

Hridayesh Prasad; Sonjoy Kumar Borthakur; Seikh Sahanawaz Alam; Gautam Patra;
Open Access English
  • Published: 01 Dec 2015 Journal: Asian Pacific Journal of Tropical Disease (issn: 2222-1808, eissn: 2222-1808, Copyright policy)
  • Publisher: Asian Pacific Journal of Tropical Disease Editorial Office
Abstract
Abstract Objective To ascertain whether immunodominant antigens of Trichuris ovis might share and cross react with host molecule. Methods Two crude protein preparations from anterior and posterior parts of Trichuris ovis were characterized along with host mucosal antigen by double immunodiffusion, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting technique. Conventional scanning electron microscopy was performed as per standard procedure. Results Sharp and distinct bands of three antigens have been found in double immunodiffusion using hyperimmune serum raised in rabbit indicating the presence of specific antibody against each antige...
Subjects
free text keywords: Trichuris ovis, Host mucosal antigens, Double immunodiffusion, SDS-PAGE, Western blot, SEM, Medicine, R, Arctic medicine. Tropical medicine, RC955-962, Biology, Virology, Cross-reactivity, medicine.disease_cause, Ouchterlony double immunodiffusion, Gel electrophoresis, Blot, Sodium, chemistry.chemical_element, chemistry, Antigen, Molecular biology, Standard procedure
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