Isolation and recrystallization of epicuticular waxes from Sorbus and Cotoneaster leaves
Ruiz Segundo Ríos
- Publisher: De Gruyter
Open Life Sciences
Rosaceae | epidermis | wax extraction | freezeembedding | recrystallization | Biology (General) | QH301-705.5
Wax morphology and chemical composition
are widely accepted to be important for the protective
properties of the leaf’s surface and also valuable
characteristics in plant systematics. The leaves of Sorbus
domestica L. and Cotoneaster granatensis Boiss., species
of two large genera with intricate taxonomy referred
to subtribe Pyrinae, Rosaceae (formerly subfamily
Maloideae), were studied by scanning electron microscope
(SEM) and performing different methods of wax isolation.
The aim of the study was to acquire a suitable, cost and
time effective method for wax removal. Chloroform and
methanol extractions and freeze-embedding method
for direct isolation of the wax crystals were applied.
Immersing the leaves for 3 minutes in chloroform was
sufficient to extract the waxes whereas the efficiency of
the methanol solvent was lower. Wax layers with wellpreserved
structures of the crystals from both upper and
lower epidermis were successfully transferred to artificial
surfaces. The recrystallization experiment demonstrated
that waxes from chloroform extracts could recrystallize
in in vitro conditions on artificial surfaces. The crystals
showed same micromorphology as on the intact leaves.
Results of this study could be applied in further analytical
researches of the waxes of S. domestica and C. granatensis
or other species of the subtribe Pyrinae.