Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions

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Elena Bobeková ; Michal Tomšovský ; Petr Horáček (2008)
  • Publisher: Mendel University Press
  • Journal: Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis (issn: 1211-8516, eissn: 2464-8310)
  • Related identifiers: doi: 10.11118/actaun200856020281
  • Subject: Agriculture | <i>Cladosporium oxysporium</i> | S | fungi | <i>Serpula lacrymans</i> | <i>Donkioporia expansa</i> | wood | <i>Amylostereum areolatum</i> | Biology (General) | QH301-705.5
    mesheuropmc: complex mixtures | technology, industry, and agriculture

The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil™ DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR–based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA.
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