publication . Article . 2013

Impact of Storage and Purification on Mitochondrial Membrane Potential of Boar Spermatozoa

Aristotelis G. Lymberopoulos; TAREK KHALIFA;
Open Access English
  • Published: 01 May 2013 Journal: Scientific Papers Animal Science and Biotechnologies, volume 46, issue 1, pages 58-64 (issn: 1841-9364, eissn: 2344-4576, Copyright policy)
  • Publisher: Agroprint Timisoara
Abstract
This study aimed to evaluate the effect of semen purification and storage on sperm mitochondrial membrane potential (ΔΨm). Gel-free whole ejaculates were collected from five proven fertile Large White boars aged two to three years. Aliquots of fresh semen were split, diluted in one step with commercial extenders and incubated at 37oC for 5-10 minutes. Semen was cooled to 18oC and packaged in 15-ml sterile propylene tubes. After 4-10 hours post-semen collection, stored semen was purified by colloidal centrifugation. After 48 hours post-semen collection, stored semen was incubated at 37oC and evaluated after 45 minutes for motility, velocity and sperm ΔΨm. Samples...
Subjects
Medical Subject Headings: endocrine systemurogenital systemfluids and secretions
free text keywords: boar, mitochondrial membrane potential, purification, sperm storage, lcsh:Agriculture, lcsh:S, lcsh:Technology, lcsh:T, lcsh:Science, lcsh:Q
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